A novel small animal model for biocompatibility assessment of polymeric materials for use in prosthetic heart valves.
A novel small animal model for biocompatibility assessment of polymeric materials for use in prosthetic heart valves.
复制标题
一种新型小动物模型，用于评估用于人工心脏瓣膜的聚合物材料的生物相容性。
DOI：
10.1002/jbm.a.32562
复制
发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Schoephoerster,RichardT
中科院分区：
文献类型：
--
作者：
Wang,Qiang;McGoron,AnthonyJ;Pinchuk,Leonard;Schoephoerster,RichardT
一种复合聚合物材料，即具有嵌入式增强聚对苯二甲酸乙二醇酯（PET）织物的聚（苯乙烯-嵌段-异丁烯-嵌段-苯乙烯）（SIBS），正在研究其在新型心脏瓣膜中的潜在用途。本研究的目的是开发和实施一种小动物模型，以评估复合材料样本在心血管组织和血液接触环境中的生物相容性。采用浸涂和溶剂浇铸两种涂层厚度（25和50 μm）制造复合材料样品。开发了一种新型大鼠腹主动脉模型，以在与其预期用途相似的脉动流条件下测试浸涂样品，并使用大鼠皮下模型测试浸涂和溶剂浇铸样品。当SIBS的光滑涂层保护PET织物免于暴露于血液和组织时，由细胞浸润和包封的程度定义的组织反应被最小化，并且组织反应的程度与表面粗糙度的程度直接相关。使用磷脂的表面改性进一步降低了组织反应。我们已经证明了使用新型大鼠腹主动脉模型对将用于心血管植入物的聚合物材料进行生物相容性评估的适用性。出于本研究的目的，我们的结果表明，具有25 μm SIBS涂层厚度的溶剂浇铸将为SIBS瓣膜提供最佳的生物相容性。© 2009威利期刊公司.生物医学材料研究杂志2010
A composite polymeric material, poly(styrene‐block‐isobutylene‐block‐styrene) (SIBS) with an embedded reinforcement polyethylene terephthalate (PET) fabric, is undergoing investigation for potential use in a novel heart valve. The purpose of this study was to develop and implement a small animal model to assess the biocompatibility of composite samples in a cardiovascular tissue and blood‐contacting environment. Composite samples were manufactured using dip coating and solvent casting with two coating thicknesses (25 and 50 μm). A novel rat abdominal aorta model was developed to test the dip‐coated samples in a similar pulsatile flow condition to its intended use, and both dip‐coated and solvent‐cast samples were tested using a rat subcutaneous model. Tissue response, defined by degree of cellular infiltration and encapsulation, was minimized when a smooth coating of SIBS shielded the PET fabric from exposure to blood and tissue, and the degree of tissue response was directly correlated with the degree of surface roughness. Surface modification using phospholipid further reduces the tissue response. We have demonstrated the applicability of using a novel rat abdominal aorta model for biocompatibility assessment of polymeric materials that will be used in cardiovascular implants. For the purpose of this study, our results indicate that solvent casting with a 25‐μm SIBS coating thickness will provide optimal biocompatibility for the SIBS valve. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res 2010