Use of wax-petrolatum layers for screening rice root penetration
Use of wax-petrolatum layers for screening rice root penetration
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使用蜡-凡士林层筛选水稻根系渗透
DOI：
10.2135/cropsci1995.0011183x003500030005x
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发表时间：
1995
期刊：
影响因子：
2.3
通讯作者：
H. Nguyen
中科院分区：
文献类型：
--
作者：
Long;J. Ray;J. O’toole;H. Nguyen
根系穿透压实土壤的能力有助于避免干旱胁迫。然而，由于缺乏有效、可靠的筛选技术，根穿透育种受到阻碍。本研究的目的是开发和评价蜡-矿脂层系统作为参考来测量11个水稻（Oryza sativa L.）来自旱地和湿地水文起源的栽培品种。蜡-矿脂层由60%蜡和40%矿脂白色组成，用土壤渗透计测量，在27 ℃下的抗渗透强度为1.4 MPa。将蜡-矿脂层置于盆中，并用作根生长的部分屏障。水稻栽培品种以随机完全区组设计播种，重复三次，并在生长室中生长。根穿透能力测量为穿透蜡-矿脂层的根的数目与根的总数的比率。因此，旱地水稻品种的根系渗透能力强于湿地水稻品种。根系渗透能力范围为6 - 26%。根系厚度在0.9 ~ 1.5mm之间，与根系穿透力呈显著正相关（r=0.81，P<0.001）。数据表明，使用石蜡-矿脂层是一种有效的技术，用于测量根穿透能力的差异
The ability of roots to penetrate compacted soils can be beneficial in avoiding drought stress. However, breeding for root penetration is hindered by the lack of an efficient, reliable screening technique. Our objectives in this study were to develop and evaluate the use of a wax-petrolatum layer system as a reference to measure genotypic variation in root penetration ability of 11 rice (Oryza sativa L.) cultivars from dryland and wetland hydrological origins. The wax-petrolatum layers consisted of 60% wax and 40% petrolatum white, with a penetration resistance strength of 1.4 MPa at 27°C as measured with a soil penetrometer. The wax-petrolatum layers were placed in pots and served as a partial barrier to root growth. Rice cultivars were sown in a randomized complete block design with three replications and grown in a growth chamber. Root penetration ability was measured as the ratio of the number of roots that penetrated the wax-petrolatum layer to the total number of roots. Consistently, rice cultivars from dryland hydrological origins had greater root penetration ability than did cultivars from wetland origins. Root penetration ability ranged from 6 to 26%. Root thickness ranged from 0.9 to 1.5 mm and was positively correlated (r=0.81, P<0.001) with root penetration ability. Data indicated that the use of wax-petrolatum layers was an effective technique for measuring differences in root penetration ability