Distinct progenitor populations in skeletal muscle are bone marrow derived and exhibit different cell fates during vascular regeneration.
Distinct progenitor populations in skeletal muscle are bone marrow derived and exhibit different cell fates during vascular regeneration.
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DOI：
10.1172/jci16157
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
S. Majka;K. Jackson;Kirsten A Kienstra;M. Majesky;M. Goodell;K. Hirschi
中科院分区：
文献类型：
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作者：
S. Majka;K. Jackson;Kirsten A Kienstra;M. Majesky;M. Goodell;K. Hirschi
血管祖细胞以前从血液和骨髓中分离，在这里，我们定义的存在，表型，潜力和起源的血管祖细胞驻留在成人骨骼肌。从后肢肌肉中同时分离出两个不同的细胞群：高度纯化的造血干细胞和非SP细胞的侧群（SP），它们不重建血液。肌肉SP细胞被认为是来自，并补充，骨髓SP细胞，然而，在肌肉环境中，他们的表型不同于骨髓SP细胞。非SP细胞也来源于骨髓干细胞，并含有具有间充质表型的祖细胞。从Rosa26小鼠中分离肌肉SP和非SP细胞，并直接注射到基因匹配受体的损伤肌肉中。血管再生过程中SP细胞移入内皮，非SP细胞移入平滑肌。因此，不同的血管祖细胞群体驻留在骨骼肌内，来源于骨髓，并且在损伤诱导的血管再生期间表现出不同的细胞命运。
Vascular progenitors were previously isolated from blood and bone marrow; herein, we define the presence, phenotype, potential, and origin of vascular progenitors resident within adult skeletal muscle. Two distinct populations of cells were simultaneously isolated from hindlimb muscle: the side population (SP) of highly purified hematopoietic stem cells and non-SP cells, which do not reconstitute blood. Muscle SP cells were found to be derived from, and replenished by, bone marrow SP cells; however, within the muscle environment, they were phenotypically distinct from marrow SP cells. Non-SP cells were also derived from marrow stem cells and contained progenitors with a mesenchymal phenotype. Muscle SP and non-SP cells were isolated from Rosa26 mice and directly injected into injured muscle of genetically matched recipients. SP cells engrafted into endothelium during vascular regeneration, and non-SP cells engrafted into smooth muscle. Thus, distinct populations of vascular progenitors are resident within skeletal muscle, are derived from bone marrow, and exhibit different cell fates during injury-induced vascular regeneration.