Sequence analysis and evolutionary perspectives of ROB-1 beta-lactamase
Sequence analysis and evolutionary perspectives of ROB-1 beta-lactamase
复制标题
ROB-1 β-内酰胺酶的序列分析和进化观点
DOI：
10.1128/aac.34.7.1354
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发表时间：
1990
影响因子：
4.9
通讯作者：
R. C. Levesque
中科院分区：
文献类型：
--
作者：
J. Juteau;R. C. Levesque
测定了流感嗜血杆菌质粒RRob的ROB-1 β-内酰胺酶基因的核苷酸序列。结构基因编码305个氨基酸的多肽，成熟形式的蛋白质的估计分子量为30，424。ROB-1基因与其他β-内酰胺酶在核酸水平上同源性较低。通过使用两种统计计算机方法，评估ROB-1和其他已知的β-内酰胺酶氨基酸序列之间的相似性程度，表明ROB-1是A类酶。A类β-内酰胺酶与ROB-1的比对鉴定了保守残基。使用突变矩阵检测距离关系表明ROB-1与革兰氏阳性菌的β-内酰胺酶具有更高的值和同源性，从而深入了解其祖先和分歧。
The nucleotide sequence of the ROB-1 beta-lactamase gene from Haemophilus influenzae plasmid RRob was determined. The structural gene encodes a polypeptide of 305 amino acids, with an estimated molecular mass of 30,424 for the mature form of the protein. The ROB-1 gene showed low homologies with other beta-lactamases at the nucleic acid level. By using two statistical computer methods, assessment of the extent of similarity between ROB-1 and other known beta-lactamase amino acid sequences suggested that ROB-1 is a class A enzyme. Alignment of class A beta-lactamases with ROB-1 identified conserved residues. The use of a mutation matrix for detecting distance relationships indicated that ROB-1 has higher values and homologies with beta-lactamases of gram-positive bacteria, giving insight into its ancestry and divergence.
DOI：
10.1073/pnas.84.14.4767
发表时间：
1987-07-01
影响因子：
11.1
作者：
TABOR, S;RICHARDSON, CC
通讯作者：
RICHARDSON, CC
影响因子：
4.1
作者：
JORIS, B;GHUYSEN, JM;KNOX, JR
通讯作者：
KNOX, JR