Calcium‐Dependent Proteases in Neuroblastoma Cells
Calcium‐Dependent Proteases in Neuroblastoma Cells
复制标题
神经母细胞瘤细胞中的钙依赖性蛋白酶
DOI：
10.1111/j.1471-4159.1982.tb06645.x
复制
发表时间：
1982
影响因子：
4.7
通讯作者：
D. E. Croall
中科院分区：
文献类型：
--
作者：
G. Demartino;D. E. Croall
翻译后摘要：神经母细胞瘤细胞的提取物含有钙依赖性蛋白水解活性。这种活性由两种不同的蛋白酶引起。通过离子交换色谱法分离这些蛋白酶。虽然每种酶都完全依赖于钙，但酶的钙需求量不同;一种酶需要约40 μM Ca 2+才能获得半最大活性，另一种酶需要约150 μM Ca 2+才能获得半最大活性。
Abstract: Extracts of neuroblastoma cells contained calcium‐dependent proteolytic activity. This activity was accounted for by two distinct proteases. These proteases were separated by ion‐exchange chromatography. Although each was totally dependent on calcium, the calcium requirements of the enzymes were different; one enzyme required approximately 40 μM Ca2+ for half‐maximal activity and the other enzyme required approximately 150 μM Ca2+ for half‐maximal activity.