Predicting oligonucleotide affinity to nucleic acid targets
Predicting oligonucleotide affinity to nucleic acid targets
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DOI：
10.1017/s1355838299991148
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发表时间：
1999-11-01
期刊：
影响因子：
4.5
通讯作者：
Turner, DH
中科院分区：
文献类型：
--
作者：
Mathews, DH;Burkard, ME;Turner, DH
报道了一种计算机程序OligoWalk，它预测互补DNA或RNA寡核苷酸对RNA靶的平衡亲和力。该程序考虑了多核苷酸-靶螺旋的预测稳定性以及与靶和寡核苷酸的预测二级结构的竞争。单分子和双分子寡核苷酸自身结构均以用户定义的浓度考虑。OligoWalk的应用说明了从文献中得出的实验结果的三个比较。
A computer program, OligoWalk, is reported that predicts the equilibrium affinity of complementary DNA or RNA oligonucleotides to an RNA target. This program considers the predicted stability of the oligonucleotide-target helix and the competition with predicted secondary structure of both the target and the oligonucleotide, Both unimolecular and bimolecular oligonucleotide self structure are considered with a user-defined concentration. The application of OligoWalk is illustrated with three comparisons to experimental results drawn from the literature.