Bystander responses in three-dimensional cultures containing radiolabelled and unlabelled human cells.
Bystander responses in three-dimensional cultures containing radiolabelled and unlabelled human cells.
复制标题
含有放射性标记和未标记人类细胞的三维培养物中旁观者的反应。
DOI：
10.1093/rpd/ncl460
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发表时间：
2006
影响因子：
1
通讯作者：
Howell,RW
中科院分区：
文献类型：
--
作者：
Pinto,M;Azzam,EI;Howell,RW
Research on the radiation-induced bystander effect has been carried out mainly in 2-D tissue culture systems. This study uses a 3-D model, wherein apparently normal human diploid fibroblasts (AG1522) are grown in a carbon scaffold, to investigate the induction of a G1checkpoint in bystander cells present alongside radiolabelled cells. Cultures were simultaneously pulse-labelled with3H-deoxycytidine (3HdC) to selectively irradiate a minor fraction of cells, and bromodeoxyuridine (BrdU) to identify the radiolabelled cells. After thorough washing of cultures, iododeoxyuridine (IdU) was administered to detect proliferating bystander cells. The cultures were harvested at various times thereafter, and cells were reacted with two monoclonal antibodies specific to IdU/BrdU or BrdU, respectively, stained with propidium iodide, and subjected to multi-parameter flow cytometry. Cell-cycle progression was followed in radiolabelled cells (BrdU+) that were chronically irradiated by low energy beta particles emitted by DNA-incorporated3H, and in unlabelled bystander cells (BrdU−) by a flow cytometry based cumulative labelling index assay. As expected, radiolabelled cells were delayed, in a dose-dependent manner, in G2and subsequently G1. No delay occurred in progression of bystander cells through G1, when the labelled cells were irradiated at dose rates up to 0.32 Gy h−1.
