INVESTIGATION OF THE ANTITRYPANOSOMAL ACTIVITY OF BUCHHOLZIA CORIACEA SEED EXTRACT AGAINST A FIELD STRAIN OF TRYPANOSOMA CONGOLENSE
INVESTIGATION OF THE ANTITRYPANOSOMAL ACTIVITY OF BUCHHOLZIA CORIACEA SEED EXTRACT AGAINST A FIELD STRAIN OF TRYPANOSOMA CONGOLENSE
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DOI：
10.4314/ajtcam.v8i5s.23
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发表时间：
2011-01-01
影响因子：
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通讯作者：
Asuzu, I. U.
中科院分区：
文献类型：
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作者：
Nweze, N. E.;Anene, B. M.;Asuzu, I. U.
用实验感染的雌雄小鼠研究了革皮Buchholzia coriacea种子甲醇提取物对刚果锥虫野外株的抗锥虫活性。寄生虫血症的监测是通过快速匹配技术。当寄生虫血症约为log 7.8（63 x 10（6）个寄生虫/ml）时，连续5天使用分级剂量的提取物（250、500和1000 mg/kg）进行治疗。二乙酰氨酰地咪嗪（Dimivet（R）SKM Pharma Pvt. Ltd.）以3.5mg/kg i. p.阳性对照小鼠。未观察到体重的显著差异。受感染小鼠的直肠温度出现波动。感染小鼠的PCV值显著低于未感染对照组（p < 0.05）。浸提液处理和未处理动物的PCV之间无显著差异。在浸提液处理和未处理小鼠组中，寄生虫血症稳步增加，直至所有动物死亡。二乙酰氨酰亚胺治疗后3天，清除了寄生虫血症。6天后，感染复发。到实验结束时，在二乙酰氨酰亚胺治疗组中记录到50%的复发率。在试验剂量下，布赫木种子的甲醇提取物在感染刚果锥虫的小鼠中未显示出任何抗锥虫活性。
The antitrypanosomal activity of the methanol extract of Buchholzia coriacea seed against a field strain of Trypanosoma congolense was investigated using experimentally infected mice of both sexes. Monitoring of parasitaemia was by the rapid matching technique. When parasitaemia was approximately log 7.8 (63 x 10(6) parasites/ml), treatment with graded doses of the extract (250, 500 and 1000 mg/kg) was instituted for 5 consecutive days. Diminazene diaceturate (Dimivet (R) SKM Pharma Pvt. Ltd.) was given at 3.5 mg/kg i. p. to the positive control mice. No significant differences in body weights were observed. The rectal temperatures of infected mice showed fluctuations. The PCV of infected mice were significantly (p < 0.05) lower than those of the uninfected controls. There was no significant difference between the PCV of the extract-treated and untreated animals. Parasitaemia increased steadily in the extract-treated and untreated mice groups till all the animals died. Three days post-treatment with diminazene diaceturate parasitaemia was cleared. Six days later, there was a relapse of infection. By the end of the experiment, a 50 % relapse rate was recorded in the diminazene diaceturate-treated group. The methanol extract of Buchholzia coriacea seeds did not show any antitrypanosomal activity in mice infected with Trypanosoma congolense at the doses tested.