Characterization of the retinoic acid receptor genes raraa, rarab and rarg during zebrafish development
Characterization of the retinoic acid receptor genes raraa, rarab and rarg during zebrafish development
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DOI：
10.1016/j.modgep.2005.10.007
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发表时间：
2006-06-01
影响因子：
1.2
通讯作者：
Postlethwait, JH
中科院分区：
文献类型：
--
作者：
Hale, LA;Tallafuss, A;Postlethwait, JH
维甲酸信号对于中枢神经系统、成对的附件、消化道和其他器官的模式是重要的。为了开始研究斑马鱼中的维甲酸信号，我们确定了斑马鱼和四足类维甲酸受体(RAR)之间的同源性，并检测了RAR基因在胚胎发育过程中的表达模式。系统发育和保守序列分析表明，所克隆的3个斑马鱼rar基因包括raraa和rarab，它们是四足动物raa的同源基因，rarg是四足动物rarg的同源基因。然而，我们没有发现Rarb的同源基因。RNA原位杂交实验表明，rarab和rarg均为母系表达。RaraA的合子表达主要发生在后脑、外侧中胚层和尾芽。Rarab的合子表达在很大程度上与Raraa重叠，只是在后期，Rarab在大脑、胸鳍芽和咽弓中更广泛地表达。斑马鱼rarg的合子表达也与其他两个基因重叠，但在体细胞发育后期开始的后脑以及咽弓的神经脊细胞中表达更强。因此，这三个基因在很大程度上具有重叠的表达模式和少数基因特异的表达结构域。对这些表达模式的了解将指导对这些基因在发育中所起作用的解释。(C)2005 Elsevier B.V.保留所有权利。
Retinoic acid signaling is important for patterning the central nervous system, paired appendages, digestive tract, and other organs. To begin to investigate retinoic acid signaling in zebrafish, we determined orthologies between zebrafish and tetrapod retinoic acid receptors (Rars) and examined the expression patterns of rar genes during embryonic development. Analysis of phylogenies and conserved syntenies showed that the three cloned zebrafish rar genes include raraa and rarab, which are co-orthologs of tetrapod Rara, and rarg, which is the zebrafish ortholog of tetrapod Rarg. We did not, however, find an ortholog of Rarb. RNA in situ hybridization experiments showed that rarab and rarg, are maternally expressed. Zygotic expression of raraa occurs predominantly in the hindbrain, lateral mesoderm, and tailbud. Zygotic expression of rarab largely overlaps that of raraa, except that in later stages rarab is expressed more broadly in the brain and in the pectoral fin bud and pharyngeal arches. Zygotic expression of zebrafish rarg also overlaps the other two genes, but it is expressed more strongly in the posterior hindbrain beginning in late somitogenesis as well as in neural crest cells in the pharyngeal arches. Thus, these three genes have largely overlapping expression patterns and a few gene-specific expression domains. Knowledge of these expression patterns will guide the interpretation of the roles these genes play in development. (c) 2005 Elsevier B.V. All rights reserved.