Clot Retraction Facilitates Clot Lysis
Clot Retraction Facilitates Clot Lysis
复制标题
凝块回缩促进凝块溶解
DOI：
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
J. M. Gilliam
中科院分区：
文献类型：
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作者：
R. Carroll;J. Gerrard;J. M. Gilliam
使用稀释凝块溶解试验研究了血小板促进凝块溶解，这是一种标准化的纤维蛋白溶解试验，显示与术后深静脉血栓形成相关。与以类似方式从稀释的富血小板血浆制备的凝块相比，从稀释的贫血小板血浆制备的凝块显示出延长的凝块溶解。由于在存在血小板的情况下发生凝块回缩或收缩，我们评估了回缩对凝块溶解的可能直接贡献。通过离心将稀释的贫血小板血浆凝块压缩至与稀释的富血小板血浆中凝块收缩期间达到的程度相似的程度。这些凝块现在以接近稀释的富含血小板的血浆凝块的速率溶解。使用另一种替代方法，用细胞松弛素B处理稀释的富含血小板的血浆凝块，以防止凝块收缩。这种凝块现在显示出与稀释的贫血小板血浆相似的延长溶解。细胞松弛素B处理的稀释的富含血小板的血浆凝块的延长的溶解通过凝块的人工压实来校正。结果表明，凝块收缩显著促进凝块溶解，并表明血小板促进凝块溶解的主要作用是这些细胞引起凝块收缩的作用。
Platelet facilitation of clot lysis was studied using the dilute clot lysis assay, a standardized assay for fibrinolysis shown to correlate with the development of postoperative deep vein thrombosis. Clots prepared from dilute platelet poor plasma showed prolonged clot lysis when compared with clots prepared in a similar fashion from dilute platelet rich plasma. Since in the presence of platelets clot retraction or contraction occurred, we evaluated a possible direct contribution of retraction to clot lysis. Dilute platelet poor plasma clots were compacted by centrifugation, to a similar extent as that achieved during clot retraction in dilute platelet rich plasma. These clots now lysed at a rate that approached that seen with dilute platelet rich plasma clots. Using an alternate alternate approach, dilute platelet rich plasma clots were treated with cytochalasin B to prevent clot retraction. Such clots now showed prolonged lysis similar to that seen with dilute platelet poor plasma. The prolonged lysis of cytochalasin B treated dilute platelet rich plasma clots was corrected by artificial compaction of the clots. The results suggest that clot retraction markedly facilitates clot lysis, and shows that a major role of platelets to facilitate clot lysis is the effect of these cells to cause clot retraction.