Depot- and sex-specific differences in human leptin mRNA expression - Implications for the control of regional fat distribution
Depot- and sex-specific differences in human leptin mRNA expression - Implications for the control of regional fat distribution
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DOI：
10.2337/diabetes.46.3.342
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发表时间：
1997-03-01
期刊：
影响因子：
7.7
通讯作者：
ORahilly, S
中科院分区：
文献类型：
--
作者：
Montague, CT;Prins, JB;ORahilly, S
具有过量腹内脂肪沉积的肥胖受试者比具有主要皮下分布的肥胖的类似超重受试者遭受更大的不良代谢后果。关于调节身体脂肪区域分布的因素知之甚少。瘦素是一种最近表征的由脂肪细胞分泌的蛋白质，似乎提供调节脂肪量的长期激素反馈信号。迄今为止，还没有系统评价人体脂肪细胞瘦素表达的位点相关差异的报道。通过定量逆转录-聚合酶链反应检测了非肥胖和轻度肥胖患者接受择期手术的网膜和皮下脂肪库中分离的脂肪细胞中瘦素mRNA的水平。在所有研究的个体中（n = 24），皮下脂肪细胞中的瘦素mRNA水平高于网膜脂肪细胞（P < 0.0001）。相反，甘油-3-磷酸脱氢酶mRNA的表达没有一致的位点特异性差异。皮下-网膜瘦素mRNA表达的比值女性（5.5 ± 1.1倍）明显高于男性（1.9 ± 0.2倍）（P < 0.02）。女性皮下脂肪细胞瘦素mRNA的表达与BMI呈显著相关（P < 0.006）。因此，瘦素mRNA似乎主要由皮下脂肪细胞表达，特别是在女性中。这些发现表明瘦素在控制脂肪组织分布和质量中可能起作用。
Obese subjects with excess intra-abdominal fat deposition suffer greater adverse metabolic consequences than do similarly overweight subjects with a predominantly subcutaneous distribution of adiposity. Little is known about the factors regulating the regional distribution of body fat. Leptin is a recently characterized protein secreted by adipocytes that appears to provide a long-term hormonal feedback signal regulating fat mass. No systematic evaluation of site-related differences in human adipocyte leptin expression has been reported to date. Levels of leptin mRNA were examined by quantitative reverse transcription-polymerase chain reaction in adipocytes isolated from omental and subcutaneous adipose depots of nonobese and mildly obese individuals undergoing elective surgery. In all individuals studied (n = 24), leptin mRNA levels were higher in subcutaneous than in omental adipocytes (P < 0.0001). In contrast, there were no consistent site-specific differences in the expression of glycerol-3-phosphate dehydrogenase mRNA. The subcutaneous-to-omental ratio of leptin mRNA expression was markedly higher in women (5.5 +/- 1.1-fold) than in men (1.9 +/- 0.2-fold) (P < 0.02). A significant relationship between BMI and leptin mRNA expression was demonstrable in the subcutaneous adipocytes of women (P < 0.006). Thus, leptin mRNA appears to be expressed predominantly by subcutaneous adipocytes, particularly in women. These findings suggest a possible role for leptin in the control of adipose tissue distribution and mass.