The ontogeny of serum GH binding protein in man: a possible indicator of hepatic GH receptor development.
The ontogeny of serum GH binding protein in man: a possible indicator of hepatic GH receptor development.
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人类血清 GH 结合蛋白的个体发育：肝 GH 受体发育的可能指标。
DOI：
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发表时间：
1987
影响因子：
5.8
通讯作者：
Brian A. Andrews
中科院分区：
文献类型：
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作者：
W. Daughaday;B. Trivedi;Brian A. Andrews
在兔的实验表明，血清生长激素结合蛋白(GH-BP)可能主要来源于肝膜上结合的GH受体。人血清含有一种特定的生长激素结合蛋白，可通过与[125I]hGH孵育和通过UltroGel ACA 44小柱凝胶过滤将[125I]hGH-BP从游离的[125I]hGH中分离出来。在每次测定中，参考(正常年轻人)血清的GH-BP活性被类似地运行，并且结果以未知血清的活性除以参考血清的GH-BP活性的百分比来表示，在校正了未知血清的GH含量导致的GH结合的预期抑制之后。11例早产儿脐血GH结合蛋白(RSGH-BP)平均为3.2+/-1.4%(SE)，17例足月脐血为14.9+/-2.5%。在生命的前20年中，RSGH-BP逐渐上升，个体差异很大。13例受试者血清RSGH-BP平均值为54.5+/-6.2%。15例青壮年血清RSGH-BP结果较为均匀，阳性率为91.7%/-7.4%。12例健康老年人(60~70岁)血清RSGH-BP降低77.2+/-5.4%。胎儿血清中RSGH-BP的低水平与已报道的绵羊和大鼠胎肝膜中GH受体的低浓度一致。我们认为GH-BP活性的测定为人类GH受体的个体发育提供了一种简单、非侵入性的测量方法。
Experiments in rabbits suggest that a serum GH binding protein (GH-BP) probably is derived largely from hepatic membrane bound GH receptors. Human serum contains a specific GH binding protein which can be easily measured by incubation with [125I] hGH and separation of [125I] hGH-BP complexes from free [125I] hGH by gel filtration through an Ultrogel AcA 44 minicolumn. In each assay GH-BP activity of a reference (normal young adult) serum is similarly run and the results are expressed as a percentage of the activity of the unknown serum divided by the GH-BP activity of the reference serum after correction for the expected inhibition of GH binding resulting from the GH content of the unknown serum. The mean relative specific GH binding protein (RSGH-BP) of cord serum from 11 premature infants was only 3.2 +/- 1.4% (SE) and of cord serum from 17 full term infants was 14.9 +/- 2.5%. During the first two decades of life there was a progressive rise of RSGH-BP with considerable individual variation. The mean serum RSGH-BP of 13 such subjects was 54.5 +/- 6.2%. More uniform RSGH-BP results were obtained in serum from 15 young adults, 91.7 +/- 7.4%. Lower RSGH-BP 77.2 +/- 5.4% was found in serum from 12 healthy older adults (age 60 to 70 years). The low levels of RSGH-BP in fetal serum are consistent with the reported low concentrations of GH receptors in sheep and rat fetal liver membranes. We suggest the measurement of GH-BP activity provides a simple, noninvasive measure of the ontogeny of GH receptors of human beings.