Two Olean-27-carboxylic Acid-Type Triterpenoids as Chemical Markers Isolated from Saxifraga umbellulata
Two Olean-27-carboxylic Acid-Type Triterpenoids as Chemical Markers Isolated from Saxifraga umbellulata
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从虎耳草中分离出两种橄榄-27-羧酸型三萜类化合物作为化学标记
DOI：
10.1002/cbdv.202100902
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Guoyue Zhong.
中科院分区：
文献类型：
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作者：
Wei Jiang;Ren Wu;Gang Ren;Yongwei Qiu;Xiaolang Du;Zejing Mu;Ting Tan;Shanshan Li;Bo Li;Wanzhi Li;Yaxin Hu;Qi Zhang;Rongrui Wei;Guoyue Zhong.
从伞蕨科（saxifraga umbellulata）中分离到两个olean - 27 -羧酸型三萜（1和2），是首次在伞蕨属（saxifraga abellulata）中发现化学成分。化合物1经综合光谱和X射线晶体学分析确定为新化合物，命名为“沙弗拉西酸”。化合物2（去乙酰化沙棘草酸）是以前报道过的已知化合物，但通过X射线晶体学分析首次描述了它的绝对构型。此外，我们还评估了它们对5种消化道人癌细胞系（BGC‐823、GBC‐SD、CCC‐9810、HT‐29和HepG2）的细胞毒性以及对CCl4诱导的L‐o2细胞损伤的肝保护活性。有趣的是，UPLC‐QTOFMS分析表明，这两种化合物可以作为鉴别小檗碱和小檗碱的化学标记物。
Two olean‐27‐carboxylic acid‐type triterpenoids (1and2) were isolated fromSaxifraga umbellulata(Saxifragaceae), representing the first case in the chemical discoveries of genusSaxifraga. Compound1was determined to be a new compound named ‘Saxifragic acid’ based on the comprehensive spectroscopic and X‐ray crystallographic analyses. Compound2(deacetylated saxifragic acid) is a known compound reported before, but its absolute configuration through X‐ray crystallographic analyses was first described here. In addition, their cytotoxicity against five digestive human cancer cell lines (BGC‐823, GBC‐SD, CCC‐9810, HT‐29, and HepG2) and hepatoprotective activity against CCl4‐induced L‐o2 cell injury in vitro were evaluated. Interestingly, UPLC‐QTOFMS analysis showed that these two compounds could be used as chemical markers to discriminate betweenS. umbellulataandS. tangutica, both of which are used for the treatment of hepatobiliary diseases in traditional Tibetan medicine.