Biofilm and cell adhesion strength on dental implant surfaces via the laser spallation technique.
Biofilm and cell adhesion strength on dental implant surfaces via the laser spallation technique.
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DOI：
10.1016/j.dental.2020.10.013
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发表时间：
2021-01
期刊：
影响因子：
--
通讯作者：
Grady ME
中科院分区：
文献类型：
--
作者：
Boyd JD;Stromberg AJ;Miller CS;Grady ME
本研究的目的是量化口腔细菌生物膜和成骨细胞样细胞单层与牙科种植体模拟表面之间的粘附强度差异，并制定一个度量标准，量化种植体表面对细菌和细胞粘附的生物相容性影响。利用激光脉冲吸收产生的高振幅短持续时间应力波，将细菌和细胞从钛基片上剥离。通过精确控制激光能量密度并对激光能量密度与外加应力进行校准，获得了变形链球菌生物膜与MG 63成骨样细胞单层在光滑和粗糙钛基底上的粘附差异。细胞粘附强度与生物膜粘附强度的比率（即，粘附指数）被确定为生物相容性评估的无量纲参数。MG 63细胞在光滑钛上测得的粘附强度为143 MPa，具有95% C.I（114，176），在光滑钛上测得的粘附强度为292 MPa，具有95% C. I（114，176）。(267，306）上。粘附强度为S.光滑钛上的变形钛为320 MPa，具有95% C. I. (304，333），并保持相对恒定在332 MPa，具有95% C. I. (324，343），在粗糙的钛上。光滑钛的计算粘附指数为0.451，95% C.I.（0.267，0.622），增加到0.876，具有95%C. I.（0.780，0.932）。激光溅射技术提供了一个平台，以检查权衡的粘附调节剂对生物膜和细胞粘附。这种权衡的特征在于粘附指数，该指数被提议用于辅助生物相容性筛选，并可能有助于改善植入结果。粘附指数用于确定促进细胞比生物膜更好粘附的表面因素。在此，粘附指数为10.1表明生物相容性良好。
The aim of this study is to quantify the adhesion strength differential between an oral bacterial biofilm and an osteoblast-like cell monolayer to a dental implant-simulant surface and develop a metric that quantifies the biocompatible effect of implant surfaces on bacterial and cell adhesion. High-amplitude short-duration stress waves generated by laser pulse absorption are used to spall bacteria and cells from titanium substrates. By carefully controlling laser fluence and calibration of laser fluence with applied stress, the adhesion difference between Streptococcus mutans biofilms and MG 63 osteoblast-like cell monolayers on smooth and rough titanium substrates is obtained. The ratio of cell adhesion strength to biofilm adhesion strength (i.e., Adhesion Index) is determined as a nondimensionalized parameter for biocompatibility assessment. Adhesion strength of 143 MPa, with a 95% C.I (114, 176), is measured for MG 63 cells on smooth titanium and 292 MPa, with a 95% C.I. (267, 306), on roughened titanium. Adhesion strength for S. mutans on smooth titanium is 320 MPa, with a 95% C.I. (304, 333), and remained relatively constant at 332 MPa, with a 95% C.I. (324, 343), on roughened titanium. The calculated Adhesion Index for smooth titanium is 0.451, with a 95% C.I. (0.267, 0.622), which increased to 0.876, with a 95% C.I. (0.780, 0.932), on roughened titanium. The laser spallation technique provides a platform to examine the tradeoffs of adhesion modulators on both biofilm and cell adhesion. This tradeoff is characterized by the Adhesion Index, which is proposed to aid biocompatibility screening and could help improve implantation outcomes. The Adhesion Index is implemented to determine surface factors that promote favorable adhesion of cells greater than biofilms. Here, an Adhesion Index ≫ 1 suggests favorable biocompatibility.