Coelenterazine distribution and luciferase characteristics in oceanic decapod crustaceans
Coelenterazine distribution and luciferase characteristics in oceanic decapod crustaceans
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海洋十足甲壳动物中腔肠素的分布和荧光素酶特征
DOI：
10.1007/bf00347123
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发表时间：
1995
期刊：
影响因子：
2.4
通讯作者：
A. Campbell
中科院分区：
文献类型：
--
作者：
C. M. Thomson;P. Herring;A. Campbell
最近在十足类动物Systellaspis debilis的发育卵中证实了Coelenterazine的生物合成。然而，腔肠净的细胞来源和在成年十足类动物中生物合成的潜力还没有得到充分的研究。我们对来自19个科和22个科的230个十足类动物的腔肠净含量进行了系统的研究。我们首次证明，在同一十足类动物中，腔肠净既负责分泌发光又负责发光体发光。与Oplophoridae的分泌性发光相关的组织(肝胰腺和胃)中的coelenterazine(平均值=2154pmoL/标本)比角质光团(平均值=8pmoL/标本)高出近3个数量级。在三个物种的发育过程中，沙丁胺酮的含量增加了2到4个数量级。Oplophoridae种含有比其他科多一个数量级的coelenterazine(平均值=154pmoL/标本)。在11种明显不发光的十足类动物中也检测到了镇痛灵(平均值为每个样本200pmoL)。弱毒沙门氏菌荧光素酶的特性使荧光素酶分析得以发展，以促进生物发光的环境控制研究。我们假设在分泌性生物发光中对腔肠净的需求超过了食物链可以同化的量。在分泌型十足类动物的生活史中，腔肠静的显著增加支持了这一假说。此外，还报道了腔肠净发光的环境控制的假定证据。
Coelenterazine biosynthesis has recently been demonstrated in the developing eggs of the decapod Systellaspis debilis. The cellular source of coelenterazine and the potential for biosynthesis in adult decapods, however, have not been fully investigated. We have conducted a systematic study of coelenterazine content in >230 individual decapods representing 19 species of Oplophoridae and 22 species from other families. We show for the first time that coelenterazine is responsible for both secretory and photophore luminescence in the same decapod. Tissues associated with secretory luminescence (hepatopancreas and stomach) in the Oplophoridae contained almost 3 orders of magnitude more coelenterazine (mean value=2154 pmol per specimen) than cuticular photophores (mean value=8 pmol per specimen). Coelenterazine content increases by 2 to 4 orders of magnitude during the development of three species of Oplophoridae. Species of Oplophoridae contain an order of magnitude more coelenterazine than those of other families (mean value=154 pmol per specimen). Coelenterazine was also detected in 11 apparently nonluminous decapod species (mean value=200 pmol per specimen). S. debilis luciferase characterisation enabled a luciferase assay to be developed to facilitate studies of the environmental control of bioluminescence. We hypothesise that the coelenterazine requirement in secretory bioluminescence exceeds that which could be assimilated from the food-chain. The significant increase of coelenterazine during the life cycle of secretory decapods supports this hypothesis. Putative evidence for environmental control of coelenterazine luminescence is also reported.