P-2 PURINOCEPTORS REGULATE SURFACTANT SECRETION FROM RAT ISOLATED ALVEOLAR TYPE-II CELLS
P-2 PURINOCEPTORS REGULATE SURFACTANT SECRETION FROM RAT ISOLATED ALVEOLAR TYPE-II CELLS
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DOI：
10.1111/j.1476-5381.1986.tb11148.x
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发表时间：
1986-11-01
影响因子：
7.3
通讯作者：
SINGLETON, FM
中科院分区：
文献类型：
--
作者：
RICE, WR;SINGLETON, FM
1用大鼠肺泡Ⅱ型细胞观察嘌呤和嘧啶类似物对肺表面活性物质分泌的影响。2 ATP以时间和剂量依赖性方式有效地刺激[3 H]-磷脂酰胆碱（[3 H]-PC）的分泌。ATP的作用在暴露一小时后就被注意到，并持续三小时。ATP诱导的[3 H]-PC分泌的EC 50（产生1/2最大反应的浓度）为100 nM。3ADP也是表面活性剂分泌的有效促泌剂，但AMP和腺苷在浓度< 250 μ M时对表面活性剂分泌没有显著影响。ADP诱导的[3 H]-PC分泌的EC 50为250 nM。4检测其他嘌呤和嘧啶核苷酸（ITP、GTP、CTP、TTP）对[~ 3 H]-PC分泌的影响。所有嘌呤和嘧啶三磷酸检查显着增强[3 H]-PC分泌，但远远低于ATP的效力。EC 50为ITP = 10 μ M; GTP = 100 μ M; CTP = 250 μ M; TTP = 100 μ M。5 8-苯基茶碱（10 μ M，一种P1-嘌呤受体拮抗剂）、普萘洛尔（100 μ M，一种β-肾上腺素受体拮抗剂），也没有吲哚美辛（10 μ M，前列腺素合成酶抑制剂）抑制ATP诱导的分离的II型细胞的[3 H]-PC分泌。6这些数据提供了通过P2-嘌呤受体调节肺泡II型细胞表面活性物质分泌的证据。
1 Rat isolated alveolar Type II cells were utilized to examine the effect of purine and pyrimidine analogues on secretion of pulmonary surfactant. 2 ATP potently stimulated [3H]-phosphatidylcholine ([3H]-PC) secretion in a time- and dose-dependent manner. The effect of ATP was noted by one hour of exposure and persisted for three hours. The EC50 (concentration producing 1/2 the maximal response) for ATP-induced [3H]-PC secretion was 100 nM. 3 ADP was also a potent secretagogue for surfactant secretion, but AMP and adenosine had no significant effect on surfactant secretion at concentrations < 250 .mu.M. The EC50 for ADP-induced [3H]-PC secretion was 250 nM. 4 Other purine and pyrimidine nucleotides (ITP, GTP, CTP, TTP) were examined for their effect on [3H]-PC secretion. All purine and pyrimidine triphosphates examined significantly augmented [3H]-PC secretion, but were much less potent than ATP. The EC50s were ITP = 10 .mu.M; GTP = 100 .mu.M; CTP = 250 .mu.M; TTP = 100 .mu.M. 5 Neither 8-phenyltheophylline (10 .mu.M, a P1-purinoceptor antagonist), propranolol (100 .mu.M, a .beta.-adrenoceptor antagonist), nor indomethacin (10 .mu.M, a prostaglandin synthetase inhibitor) inhibited ATP-induced [3H]-PC secretion from isolated Type II cells. 6 These data provide evidence for regulation of surfactant secretion from alveolar Type II cells by a P2-purinoceptor.