Improved neural microcirculation and regeneration after peripheral nerve decompression in DPN rats
Improved neural microcirculation and regeneration after peripheral nerve decompression in DPN rats
复制标题
DPN 大鼠周围神经减压后神经微循环和再生的改善
DOI：
10.1080/01616412.2017.1297557
复制
发表时间：
2017
影响因子：
1.9
通讯作者：
Liao Chenlong
中科院分区：
文献类型：
--
作者：
Zhong Wenxiang;Yang Min;Zhang Wenchuan;Visocchi Massimiliano;Chen Xiangjun;Liao Chenlong
目的：近年来，神经微循环和再生被认为是糖尿病周围神经病变(DPN)改善的关键因素。本研究旨在探讨周围神经减压损伤神经的细胞学和分子机制。方法：雄性SD大鼠45只建立DPN模型。用HE染色观察微血管的形态和分布。用透射电子显微镜观察雪旺细胞的形态和分布。免疫组织化学染色检测神经生长因子(NGF)、酪氨酸激酶受体A(TrkA)和生长相关蛋白43(GAP-43)在坐骨神经远端的表达。结果：DPN组大鼠坐骨神经远端微血管和雪旺细胞的分布减少(p<0.05)。神经生长因子、TrkA和GAP-43在DPN组也显著降低(p<；P<；0.05)。减压组神经生长因子、TrkA、GAP-43表达增加，微血管和雪旺细胞分布增加(p<；P<0.05)。讨论：DPN大鼠神经受压后，微循环障碍和缺氧缺血，导致NGF、TrkA和GAP-43表达减少。最后，受压神经的自我修复功能受到影响。相反，神经减压术可以改善神经微循环和神经再生，改变原有的病理过程。
Objective: Recently, neural microcirculation and regeneration were regarded as critical factors in diabetic peripheral neuropathy (DPN) improvement. In the present study, we explored the cytological and molecular mechanisms how peripheral nerve decompression impaired nerve injury.Methods: Forty-five male SD rats were established as the DPN model. HE staining was used to observe the morphology and distribution of microvessels. Transmission electron microscopy was applied to observe the morphology and distribution of Schwann cells. Immunohistochemical staining was performed to measure nerve growth factor (NGF), tyrosine kinase receptor A (TrkA) and growth-associated protein 43 (GAP-43) in the distal sciatic nerve.Results: Distribution of microvessels and Schwann cells decreased in the DPN group (p< 0.05). NGF, TrkA and GAP-43 also decreased significantly in the DPN group (p< 0.05). NGF, TrkA, GAP-43 and distribution of microvessels and Schwann cells increased in the decompressed group (p< 0.05).Discussion: In DPN rats, after nerves are compressed, microcirculation disturbance and hypoxia ischemia will happen, which cause decreased expression of NGF, TrkA and GAP-43. Finally, the self-healing function of compressed nerves is impacted. Conversely, nerve decompression can improve neural microcirculation and regeneration and change the former pathological process.