Enzyme and protein mass transfer coefficient in aqueous two-phase systems—I. Spray extraction columns
Enzyme and protein mass transfer coefficient in aqueous two-phase systems—I. Spray extraction columns
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水两相体系中酶和蛋白质的传质系数——喷雾萃取柱
DOI：
10.1016/0009-2509(92)80200-v
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发表时间：
1992
影响因子：
4.7
通讯作者：
S. Sikdar
中科院分区：
文献类型：
--
作者：
K. Jafarabad;S. B. Sawant;J. Joshi;S. Sikdar
在22、34、50、56、70和95 mm i. d.内测定了牛血清白蛋白（BSA）和淀粉葡糖苷酶的分散相滞留分数和分散侧传质系数。使用盐-聚乙二醇（磷酸钾-PEG和硫酸钠-PEG）系统的喷雾柱。考察了分布器设计和塔高对分离效果的影响。研究了水相体系的相组成对反应的影响。经验和半经验的关联式已经开发的分数分散相滞留和分散侧传质系数。
Fractional dispersed phase hold-up and dispersed side mass transfer coefficients for bovine serum albumin (BSA) and amyloglucosidase were measured in 22, 34, 50, 56, 70 and 95 mm i.d. spray columns using salt-polyethylene glycol (potassium phosphate-PEG and sodium sulphate-PEG) systems. The effect of distributor design and column height were investigated. The effect of phase compositions of the aqueous phase system was also studied. Empirical and semi-empirical correlations have been developed for fractional dispersed phase hold-up and dispersed side mass transfer coefficients.