Comparison of cytochrome P-450 species which catalyze the hydroxylations of the aromatic ring of estradiol and estradiol 17-sulfate
Comparison of cytochrome P-450 species which catalyze the hydroxylations of the aromatic ring of estradiol and estradiol 17-sulfate
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催化雌二醇和雌二醇17-硫酸酯芳环羟基化的细胞色素P-450种类的比较
DOI：
10.1016/0960-0760(91)90087-l
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
I. Yoshizawa
中科院分区：
文献类型：
--
作者：
Kazuhiro Watanabe;K. Takanashi;S. Imaoka;Y. Funae;S. Kawano;Katsuhiro Inoue;T. Kamataki;H. Takagi;I. Yoshizawa
For identification of microsomal cytochrome P-450 (P-450) enzymes which catalyze 2- or 4-hydroxylations of estrogens in the rat liver, estradiol (E2) and estradiol 17-sulfate (E2-17-S) were selected as the substrates and incubated with various kinds of purified P-450 enzymes: PB-1, PB-2, PB-4 and PB-5 obtained from phenobarbital-treated male rats (Sprague-Dawley); MC-1 and MC-5 from 3-methylcholanthrene-treated male rats; and UT-1, UT-2, UT-4 and UT-5 from untreated animals. The reactions were carried out under the P-450-reconstructed system, and the resulting products were determined by HPLC using electrochemical detection. All the enzymes tested were shown to have varying degrees of catalytic activities for 2-hydroxylation of the two substrates; UT-1 and UT-2 had the highest activity. Of the induced P-450 enzymes, PB-2 and MC-1 showed fairly high catalytic activity for 4-hydroxylation of E2. The P-450 enzymes obtained from the untreated male rats, especially UT-4, showed the highest catalytic activity for 4-hydroxylation of the two substrates. From these results and also from kinetic experiments, the P-450 enzymes which catalyze 2- and 4-hydroxylations of estrogen were considered to be different species. A part of E2was converted to such metabolites as estrone and those having a hydroxyl group at positions 6β, 15α or 16α, each production of which was estimated to be catalyzed by single or multiple P-450s.
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来自成年雄性大鼠肝微粒体的三种高度纯化的细胞色素 P-450 的表征。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Ryan,DE;Iida,S;Wood,AW;Thomas,PE;Lieber,CS;Levin,W
通讯作者：
Levin,W
人类雌酮和雌二醇的差异羟基化。
DOI：
10.1210/jcem-62-1-170
发表时间：
1986
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
Hershcopf,RJ;Bradlow,HL;Fishman,J
通讯作者：
Fishman,J
大鼠肝细胞色素 P-450 催化的 17 β-雌二醇 2- 和 4- 羟基化：个体形式的作用、诱导效应、发育模式以及性腺切除术和激素替代的改变。
影响因子：
4.8
作者：
Dannan,GA;Porubek,DJ;Nelson,SD;Waxman,DJ;Guengerich,FP
通讯作者：
Guengerich,FP
细胞色素 P-450C-M/F，雄性和雌性大鼠肝脏中微粒体细胞色素 P-450 的新组成形式，具有雌激素 2- 和 16 α-羟化酶活性。
影响因子：
2.9
作者：
Sugita,O;Sassa,S;Miyairi,S;Fishman,J;Kubota,I;Noguchi,T;Kappas,A
通讯作者：
Kappas,A