Visualization of mPer1 transcription in vitro:: NMDA induces a rapid phase shift of mPer1 gene in cultured SCN
Visualization of mPer1 transcription in vitro:: NMDA induces a rapid phase shift of mPer1 gene in cultured SCN
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DOI：
10.1016/s0960-9822(01)00445-6
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发表时间：
2001-10-02
期刊：
影响因子：
9.2
通讯作者：
Okamura, H
中科院分区：
文献类型：
--
作者：
Asai, M;Yamaguchi, S;Okamura, H
许多生理和行为现象是由一个内部的，自我维持的振荡器控制的，周期约为24小时。在哺乳动物中，主振荡器位于视交叉上核（SCN）。主观夜间的光脉冲通过SCN的直接神经兴奋性视网膜传入引起昼夜节律的相移[1]。沿着公认的生物钟理论模型，认为哺乳动物的行为重置在2小时内完成[2];然而，分子机制尚未阐明。在这里，我们显示了通过使用转基因的培养SCN中的生物钟基因mPer 1的转录的实时图像。携带mPer 1启动子控制下的荧光素酶报告基因的小鼠[3]。实时图像表明，mPer 1启动子活性以昼夜节律的方式强烈振荡，并且当发生相移时，该启动子活性迅速重置（在2-3小时内）。
Many physiological and behavioral phenomena are controlled by an internal, self-sustaining oscillator with a periodicity of approximately 24 hr. In mammals, the principal oscillator resides in the suprachiasmatic nucleus (SCN). A light pulse during the subjective night causes a phase shift of the circadian rhythm via direct glutamatergic retinal afferents to the SCN [1]. Along with the accepted theoretical models of the clock, it is suggested that behavioral resetting of mammals is completed within 2 hr [2]; however, the molecular mechanism has not been elucidated. Here, we show the realtime image of the transcription of the circadian-clock gene mPer1 in the cultured SCN by using the transgenic. mice that carry a luciferase reporter gene under the control of the mPer1 promoter [3]. The real-time image demonstrates that the mPer1 promoter activity oscillates robustly in a circadian manner and that this promoter activity is reset rapidly (within 2-3 hr) when a phase shift occurs.