Fluorogenic Resveratrol-Confined Graphene Oxide For Economic and Rapid Detection Of Alzheimer's Disease
Fluorogenic Resveratrol-Confined Graphene Oxide For Economic and Rapid Detection Of Alzheimer's Disease
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DOI：
10.1021/am5010909
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发表时间：
2014-04-23
影响因子：
9.5
通讯作者：
Tian, He
中科院分区：
文献类型：
--
作者：
He, Xiao-Peng;Deng, Qiong;Tian, He
开发一种有效的方法来实时探测与阿尔茨海默病（AD）密切相关的淀粉样蛋白β（A β），可以帮助更好地了解和监测这种疾病。在这里，我们描述了一种基于天然产物白藜芦醇（Res）与氧化石墨烯（GO）的简单组合物的经济方法，用于A β的快速荧光识别。Res@GO复合材料已被证明对一系列蛋白质和离子的A β具有特异性，并且可以在生理缓冲溶液中仅在3分钟内灵敏地捕获A β单体和纤维。该复合材料还可以在30分钟内对小鼠脑切片中的淀粉样蛋白沉积物进行荧光成像。这种新方案比临床上使用的常规免疫荧光染色技术便宜得多，为AD的简明检测提供了一种经济的工具。
Developing an effective means for the real-time probing of amyloid beta (A beta) that is closely implicated in Alzheimer's disease (AD) could help better understand and monitor the disease. Here we describe an economic approach based on the simple composition of a natural product, resveratrol (Res), with graphene oxide (GO) for the rapid, fluorogenic recognition of A beta. The Res@GO composite has proved specific for A beta over a range of proteins and ions, and could sensitively capture both A beta monomers and fibers in a physiological buffer solution within only 3 min. The composite can also fluorescently image amyloid deposits in a mouse brain section within 30 min. This new protocol is much cheaper and more timesaving than the conventional immunofluorescence staining technique employed clinically, providing an economic tool for the concise detection of AD.