Identification and pathogenicity of Pseudomonas fluorescens associated with canker disease of kiwifruit in central China
Identification and pathogenicity of Pseudomonas fluorescens associated with canker disease of kiwifruit in central China
复制标题
华中地区猕猴桃溃疡病荧光假单胞菌的鉴定及致病性
DOI：
10.1080/14620316.2014.11513058
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发表时间：
2014-01
期刊：
影响因子：
--
通讯作者：
Tan, G. J.
中科院分区：
文献类型：
--
作者：
Zhang, L. X.;Li, S. S.;He, T.;Tan, G. J.
猕猴桃细菌性溃疡病（Actinidia sp.）在世界范围内造成重大损失，以及假单胞菌pv.已知放线菌素（Psa）是该疾病的主要病原体。2010年和2011年，在中国中部安徽省进行的调查期间，在猕猴桃植物中观察到一种新的溃疡病。这种疾病的症状与其他地方通常在PSA感染的植物上观察到的症状不同。结果表明，从猕猴桃病株茎和叶片中分离到136株细菌，经鉴定为荧光假单胞菌（P. fluorescens）和拉恩氏菌（Rahnella spp.）根据生理生化试验、16 S rRNA和16 S-23 S rRNA内转录间隔区序列分析以及脂肪酸甲酯分析，荧光假单胞菌是最常见的分离种（88.2%）。以13个代表性的荧光假单胞菌和拉氏菌为材料，对金猕猴桃离体茎段和红阳猕猴桃2年生健康盆栽植株进行了致病性测定。荧光假单胞菌在猕猴桃离体茎上引起褐变坏死，在盆栽苗枝条上引起腐烂，表明荧光假单胞菌对猕猴桃茎具有致病性。然而，Rahnella spp.在猕猴桃茎上不会引起症状。据我们所知，这些结果首次表明，荧光假单胞菌可能参与猕猴桃细菌性溃疡病。
Summary Bacterial canker disease of kiwifruit (Actinidia sp.) causes significant losses worldwide, and Pseudomonas syringae pv. actinidiae (Psa) is known to be the main causal agent of the disease. In 2010 and in 2011, a new canker disease was observed in kiwifruit plants during surveys carried out in Anhui Province in central China. This disease had symptoms that were different from those usually observed elsewhere on Psa-infected plants. One hundred and thirty-six bacterial strains were obtained from the infected canes and leaves of these kiwifruit plants, and identified as P. fluorescens and Rahnella spp. based on a combination of physiological and biochemical tests, sequence analyses of their 16S rRNAs and 16S - 23S rRNA internal transcribed spacers, as well as fatty acid methyl ester analysis. P. fluorescens was the most frequently isolated species (88.2%). Pathogenicity assays were performed on detached stems of ‘Jinkwi’ kiwifruit and in 2-year-old healthy potted plants of ‘Hongyang’ kiwifruit, using 13 representative field isolates of P. fluorescens and Rahnella spp. Isolates of P. fluorescens caused brown-to-dark necrotic lesions on the detached stems, and produced rot symptoms on the twigs of potted seedlings within 2 weeks following wound-inoculation, indicating that P. fluorescens was pathogenic on kiwifruit stems. However, strains of Rahnella spp. did not induce symptoms on kiwifruit stems. To our knowledge, these results indicate, for the first time, that P. fluorescens may be involved in bacterial canker disease in kiwifruit.
DOI：
10.7326/0003-4819-111-5-448_7
发表时间：
1989-05
期刊：
--
影响因子：
--
作者：
in chief George M. Garrity
通讯作者：
in chief George M. Garrity
DOI：
10.1002/9780470168011.ch1
发表时间：
2007-01-01
期刊：
Horticultural Reviews
影响因子：
--
作者：
Ferguson, A. Ross;Huang, Hong-wen;Huang, H. W.
通讯作者：
Huang, H. W.