Allozyme variability and phylogenetic relationships in honey bee (Hymenoptera: Apidae:Apis mellifera) populations from Greece and Cyprus
Allozyme variability and phylogenetic relationships in honey bee (Hymenoptera: Apidae:Apis mellifera) populations from Greece and Cyprus
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DOI：
10.1007/s10528-005-8163-2
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发表时间：
2005-10-01
影响因子：
2.4
通讯作者：
Alahiotis, S
中科院分区：
文献类型：
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作者：
Bouga, M;Kilias, G;Alahiotis, S
从意大利蜜蜂A.M.cecropia(Pthiitida，KyThria)、A.M.macedonica(马其顿)和与A.M.Adami(Ikaria，Kasos)非常相似的爱琴海小种(A.M.Adami，Ikaria，Kasos)三个亚种的五个希腊种群中，分析了12个遗传座位对应的10个基因酶系统(α-GPDH、AO、MDH、ADH、LAP、SOD、ALP、ACPH、ME和EST)，以及来自Cypus(A.M.a)的种群。ADH*-1、ADH*-2和LAP*电泳谱将塞浦路斯人与希腊人区分开来。MDH*-1、EST*-3、SOD*、ALP*和ME*基因座在几乎所有群体中都是多态的。观察到的杂合度在0.066到0.251之间。所有座位的等位基因频率被用来估计Nei的遗传距离，在所研究的群体中，该距离在0.011到0.413之间。由遗传距离矩阵法得到的UPGMA和邻接系统发育树，以及基于离散特征简约法的Wagner树，支持最远的种群是来自塞浦路斯的种群的假设。我们的等位酶数据支持A.M.塞浦路斯作为一个独特的亚种，但没有等位酶支持存在于希腊的其他亚种的区别。
Ten gene enzymic systems (alpha-GPDH, AO, MDH, ADH, LAP, SOD, ALP, ACPH, ME, and EST), corresponding to 12 genetic loci, were assayed from five Greek populations representing three subspecies of Apis mellifera, A. m. cecropia (Pthiotida, Kythira), A. m. macedonica (Macedonia), and the "Aegean race" of A. mellifera, which is supposed to be very similar to A. m. adami (Ikaria, Kasos), as well as a population from Cypus (A. m. cypria). ADH*-1, ADH*-2, and LAP* electrophoretic patterns discriminate the Cyprus population from the Greek populations. MDH*-1, EST*-3, SOD*, ALP*, and ME* loci were found to be polymorphic in almost all populations. The observed heterozygosity was found to range from 0.066 to 0.251. Allele frequencies of all loci were used to estimate Nei's genetic distance, which was found to range between 0.011 and 0.413 among the populations studied. UPGMA and neighbor-joining phylogenetic trees obtained by genetic distance matrix methods, as well as a Wagner tree based on the discrete character parsimony method, support the hypothesis that the most distant population is that from Cyprus. Our allozymic data support A. m. cypria as a distinct subspecies, but there was no allozymic support for the distinction of the other subspecies existing in Greece.