Target-Responsive "Sweet" Hydrogel with Glucometer Readout for Portable and Quantitative Detection of Non-Glucose Targets
Target-Responsive "Sweet" Hydrogel with Glucometer Readout for Portable and Quantitative Detection of Non-Glucose Targets
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DOI：
10.1021/ja3114714
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发表时间：
2013-03-13
影响因子：
15
通讯作者：
Yang, Chaoyong James
中科院分区：
文献类型：
--
作者：
Yan, Ling;Zhu, Zhi;Yang, Chaoyong James
便携式仪器具有快速、现场、方便、经济的优点，在日常生活中得到了广泛的应用。然而，目前市面上的定量便携式设备数量有限，其中个人血糖仪（personal glucose meter， PGM）是最成功的例子，得到了最广泛的应用。然而，pgm只能检测血糖作为唯一的目标。在这里，我们描述了一种新的设计，将葡萄糖淀粉酶捕获的适体交联水凝胶与PGM相结合，用于便携式和定量检测非葡萄糖靶标。引入目标后，水凝胶分解释放葡萄糖淀粉酶，葡萄糖淀粉酶催化直链淀粉水解，产生大量葡萄糖供PGM定量读出。该方法具有成本低、快速、便携和易于使用的优点，有可能被公众用于广泛的非葡萄糖目标的便携和定量检测。
Portable devices with the advantages of rapid, on-site, user-friendly, and cost-effective assessment are widely applied in daily life. However, only a limited number of quantitative portable devices are commercially available, among which the personal glucose meter (PGM) is the most successful example and has been the most widely used. However, PGMs can detect only blood glucose as the unique target. Here we describe a novel design that combines a glucoamylase-trapped aptamer-cross-linked hydrogel with a PGM for portable and quantitative detection of non-glucose targets. Upon target introduction, the hydrogel collapses to release glucoamylase, which catalyzes the hydrolysis of amylose to produce a large amount of glucose for quantitative readout by the PGM. With the advantages of low cost, rapidity, portability, and ease of use, the method reported here has the potential to be used by the public for portable and quantitative detection of a wide range of non-glucose targets.