The mechanics of translocation: a molecular "spring-and-ratchet" system.
The mechanics of translocation: a molecular "spring-and-ratchet" system.
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DOI：
10.1016/j.str.2008.04.001
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发表时间：
2008-05
期刊：
影响因子：
--
通讯作者：
Gilbert RJ
中科院分区：
文献类型：
--
作者：
Moran SJ;Flanagan JF 4th;Namy O;Stuart DI;Brierley I;Gilbert RJ
The translation of genetic information into proteins is a fundamental process of life. Stepwise addition of amino acids to the growing polypeptide chain requires the coordinated movement of mRNA and tRNAs through the ribosome, a process known as translocation. Here, we review current understanding of the kinetics and mechanics of translocation, with particular emphasis on the structure of a functional mammalian ribosome stalled during translocation by an mRNA pseudoknot. In the context of a pseudoknot-stalled complex, the translocase EF-2 is seen to compress a hybrid-state tRNA into a strained conformation. We propose that this strain energy helps overcome the kinetic barrier to translocation and drives tRNA into the P-site, with EF-2 biasing this relaxation in one direction. The tRNA can thus be considered a molecular spring and EF-2 a Brownian ratchet in a “spring-and-ratchet” system within the translocation process.
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DOI：
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发表时间：
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期刊：
NATURE STRUCTURAL BIOLOGY
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DOI：
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发表时间：
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影响因子：
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