Dynamic analysis of Ca²+ level during bovine oocytes maturation and early embryonic development.
Dynamic analysis of Ca²+ level during bovine oocytes maturation and early embryonic development.
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DOI：
10.4142/jvs.2011.12.2.133
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发表时间：
2011-06
影响因子：
1.8
通讯作者：
Ma YH
中科院分区：
文献类型：
--
作者：
Liang SL;Zhao QJ;Li XC;Jin YP;Wang YP;Su XH;Guan WJ;Ma YH
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Mammalian oocyte maturation and early embryo development processes are Ca2+-dependent. In this study, we used confocal microscopy to investigate the distribution pattern of Ca2+ and its dynamic changes in the processes of bovine oocytes maturation, in vitro fertilization (IVF), parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) embryo development. During the germinal vesicle (GV) and GV breakdown stage, Ca2+ was distributed in the cortical ooplasm and throughout the oocytes from the MI to MII stage. In IVF embryos, Ca2+ was distributed in the cortical ooplasm before the formation of the pronucleus. In 4-8 cell embryos and morulas, Ca2+ was present throughout the blastomere. In PA embryos, Ca2+ was distributed throughout the blastomere at 48 h, similar to in the 4-cell and 8-cell phase and the morula. At 6 h after activation, there was almost no distribution of Ca2+ in the SCNT embryos. However, Ca2+ was distributed in the donor nucleus at 10 h and it was distributed throughout the blastomere in the 2-8 cell embryos. In this study, Ca2+ showed significant fluctuations with regularity of IVF and SCNT groups, but PA did not. Systematic investigation of the Ca2+ location and distribution changes during oocyte maturation and early embryo development processes should facilitate a better understanding of the mechanisms involved in oocyte maturation, reconstructed embryo activation and development, ultimately improving the reconstructed embryo development rate.
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影响因子：
6.1
作者：
Mitalipov, S. M.;Zhou, Q.;Wolf, D. P.
通讯作者：
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影响因子：
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Ducibella, T
DOI：
10.1006/bbrc.1996.0105
发表时间：
1996-01-17
影响因子：
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作者：
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