Changes in carbohydrate metabolism in Citrus sinensis infected with 'Candidatus Liberibacter asiaticus'
Changes in carbohydrate metabolism in Citrus sinensis infected with 'Candidatus Liberibacter asiaticus'
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DOI：
10.1111/j.1365-3059.2010.02328.x
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发表时间：
2010-12-01
期刊：
影响因子：
2.7
通讯作者：
Li, Z. -G.
中科院分区：
文献类型：
--
作者：
Fan, J.;Chen, C.;Li, Z. -G.
研究了甜橙子感染黄龙病病原菌“黄龙病黄龙杆菌"后碳水化合物代谢的变化。与健康对照相比，在有和没有症状的HL B感染的叶片中淀粉水平分别增加了3个中心点1倍和7个中心点9倍。在无叶的叶片中，蔗糖和果糖在中脉和叶中显著积累（P < 0中心点05），而葡萄糖仅在中脉中积累（大于5倍）;而麦芽糖水平在中脉和叶中分别降低到健康叶片中的值的64中心点6%和86中心点8%。在有症状的叶片中，与健康叶片相比，蔗糖和葡萄糖保持在高水平，同时没有观察到果糖的积累;相比之下，麦芽糖含量降低到健康叶片中的49中心点6%。四倍诱导细胞壁结合的转化酶活性检测在两种类型的叶片上患病的植物。此外，淀粉降解基因的表达谱表明，DPE 2和MEX 1的转录下调。与麦芽糖含量的减少一起，说明淀粉分解的障碍可能是导致淀粉积累的原因。碳水化合物分配的不平衡及其与疾病生理的关系进行了讨论。
Changes in carbohydrate metabolism in sweet orange (Citrus sinensis) infected with 'Candidatus Liberibacter asiaticus', a purported cause of citrus Huanglongbing (HLB), were investigated. Starch levels in HLB-infected leaves with and without symptoms increased 3 center dot 1- and 7 center dot 9-fold, respectively, compared to healthy controls. In symptomless leaves, sucrose and fructose accumulated significantly (P < 0 center dot 05) in both midribs and lobes, and glucose only in the midribs (greater than fivefold); whereas maltose levels were reduced to 64 center dot 6% and 86 center dot 8% in the midribs and foliar lobes, respectively, of the values in healthy leaves. In leaves with symptoms, sucrose and glucose remained at high levels compared to healthy leaves, whilst no accumulation of fructose was observed; by contrast, the maltose content decreased to as low as 49 center dot 6% of that in healthy leaves. Fourfold induction of cell-wall-bound invertase activity was detected in both types of leaves on diseased plants. Additionally, the expression profiles of starch breakdown genes suggested that the transcription of DPE2 and MEX1 was down-regulated. Together with the reduction of maltose, it is suggested that the impairment of starch breakdown may contribute to the starch accumulation in infected leaves. The imbalance of carbohydrate partitioning and its relation to disease physiology are discussed.