STAGE-SPECIFIC AND TISSUE-SPECIFIC EXPRESSION OF 2 HOMEO BOX GENES IN SEA-URCHIN EMBRYOS AND ADULTS
STAGE-SPECIFIC AND TISSUE-SPECIFIC EXPRESSION OF 2 HOMEO BOX GENES IN SEA-URCHIN EMBRYOS AND ADULTS
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DOI：
10.1093/nar/16.24.11543
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发表时间：
1988-12-23
影响因子：
14.9
通讯作者：
HUMPHREYS, T
中科院分区：
文献类型：
--
作者：
DOLECKI, GJ;WANG, G;HUMPHREYS, T
我们报告从夏威夷海胆 Tripneustes gratilla 中分离出两种不同的同源盒基因 HB3 和 HB4。 DNA 测序揭示了每个基因中明确的触角足 (Antp) 类同源盒。 Southern转移杂交显示这些基因是单拷贝的。在卵巢、睾丸、小肠和原肠胚 Poly(A)+ RNA 中发现了 HB3 基因的 5.7-kb 转录本。 HB4 基因产生三个转录本。 3.7-kb 和 4.4-kb 转录本在胚胎发生过程中表达。所研究的每个成体组织中均出现 3.5 kb 的转录本。 HB4 基因似乎是海胆果蝇 infrabdominal-7 (iab-7) 基因、小鼠 Hox 1.7 和 Hox 3.2 基因以及爪蟾 X1Hbox 6 基因的同源基因。对后口动物中 Antp 类同源框基因的检查表明，在棘皮动物分化后导致脊椎动物的进化谱系中发生了染色体复制。因此，海胆代表了原始状态。
We report the isolation of two different homeo box genes, HB3 and HB4, from the Hawaiian sea urchin Tripneustes gratilla. DNA sequencing revealed a definitive Antennapedia (Antp) class homeo box in each gene. Southern transfer hybridizations showed the genes to be single-copy. A 5.7-kb transcript of the HB3 gene was found in ovary, testis, small intestine and gastrula poly(A)+ RNA. The HB4 gene produces three transcripts. a 3.7-kb and a 4.4-kb transcript are expressed during embryogenesis. A 3.5-kb transcript appears in each of the adult tissues studied. The HB4 gene appears to be the sea urchin cognate of the Drosophila infrabdominal-7 (iab-7) gene, the mouse Hox 1.7 and Hox 3.2 genes and the Xenopus X1Hbox 6 gene. An examination of Antp class homeo box genes in deuterostomes indicates that a chromosomal duplication has taken place in the evolutionary line leading to the vertebrates after the divergence of the echinoderms. Thus, the sea urchin represents the primitive condition.