Investigation of the impact of the common animal facility contaminant murine norovirus on experimental murine cytomegalovirus infection
Investigation of the impact of the common animal facility contaminant murine norovirus on experimental murine cytomegalovirus infection
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DOI：
10.1016/j.virol.2009.05.035
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发表时间：
2009-09-30
期刊：
影响因子：
3.7
通讯作者：
Hill, Ann B.
中科院分区：
文献类型：
--
作者：
Doom, Carmen M.;Turula, Holly M.;Hill, Ann B.
鼠诺如病毒（MNV）是最近发现的病原体，已成为无特定病原体小鼠群体的常见污染物。MNV-1在一些小鼠品系中诱导强烈的干扰素-β反应并引起组织病理学，表明它可能影响其他小鼠感染模型。尽管对MNV-1污染有许多担忧，但关于其对其他感染的免疫反应的影响的信息很少。本研究旨在探讨MNV-1感染是否对小鼠巨细胞病毒（MCMV）感染模型产生影响。暴露于MNV-1导致BALB/c和C57 BL/6小鼠中CD 8 T细胞对免疫显性MCMV表位的应答降低。然而，MNV-1不影响任一小鼠品系中的MCMV滴度，也不刺激潜伏MCMV的再活化。这些数据表明，虽然MNV-1对MCMV的免疫应答有轻微影响，但不太可能影响MCMV模型中免疫活性小鼠的大多数实验结果。(C)2009 Elsevier Inc，保留所有权利。
Murine norovirus (MNV) is a recently discovered pathogen that has become a common contaminant of specific pathogen-free mouse colonies. MNV-1 induces a robust interferon-beta response and causes histopathology in some mouse strains, suggesting that it may impact other Mouse models of infection. Despite many concerns about MNV-1 contamination, there is little information about its impact on immune responses to other infections. This study addresses whether MNV-1 infection has an effect on a model Of murine cytomegalovirus (MCMV) infection. Exposure to MNV-1 resulted in a decreased CD8 T cell response to immunodominant MCMV epitopes in both BALB/c and C57BL/6 mice. However, MNV-1 did not impact MCMV titers in either mouse strain, nor did it stimulate reactivation of latent MCMV. These data Suggest that while MNV-1 has a mild impact on the immune response to MCMV, it is not likely to affect most experimental Outcomes in immunocompetent mice in the MCMV model. (C) 2009 Elsevier Inc, All rights reserved.