Strategies for optimizing heterologous protein expression in Escherichia coli
Strategies for optimizing heterologous protein expression in Escherichia coli
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DOI：
10.1016/s0167-7799(97)01155-4
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发表时间：
1998-02-01
影响因子：
17.3
通讯作者：
Makrides, SC
中科院分区：
文献类型：
--
作者：
Hannig, G;Makrides, SC
大肠杆菌的诸多优势确保其仍然是高效、经济、高水平生产异源蛋白质的有价值的宿主。在这里，我们描述了这种原核表达系统的现状，并重点关注旨在最大化重组蛋白产量的策略。还概述了该表达系统面临的主要挑战。
The many advantages of Escherichia coli have ensured that it remains a valuable host for the efficient, cost-effective and high-level production of heterologous proteins. Here, we describe the current status of this prokaryotic expression system and focus on strategies designed to maximize the yields of recombinant proteins. Major challenges facing this expression system are also outlined.