Changes in Bone Quality Associated with the Mineralization of New Bone Formed Around Implants - Using XPS, Polarized Microscopy, and FTIR imaging
Changes in Bone Quality Associated with the Mineralization of New Bone Formed Around Implants - Using XPS, Polarized Microscopy, and FTIR imaging
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DOI：
10.2485/jhtb.19.101
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发表时间：
2010-10-01
影响因子：
0.4
通讯作者：
LeGeros, Racquel Z.
中科院分区：
文献类型：
--
作者：
Nakada, Hiroshi;Numata, Yasuko;LeGeros, Racquel Z.
本研究的目的是研究骨质量的变化与种植体周围新骨形成的矿化有关。实验选用18周龄雄性新西兰大白兔8只，植入物由Ti-15%Zr-4%Nb-4% Ta合金制成。采用x射线光电子能谱（XPS）进行元素定性和定量分析，偏光显微镜（PM）进行组织学检查，傅里叶变换红外成像（FTIR）表征新骨和皮质骨的分子分布，比较种植体四周4周和8周形成的新骨和皮质骨的特征。XPS分析结果显示新骨化学键随时间的变化（4周vs. 8周）。PM在新骨中显示胶原双折射、取向和强度随时间的变化，8周后与皮质骨相似。FTIR成像显示，在靠近皮质骨的新骨中，PO43-和CO32-离子（代表矿物或无机成分）的量以及酰胺I（代表有机成分）的量随着矿化的进展而增加。在离皮质骨较远的新骨中，PO43-、CO32-和酰胺I的分布也随着时间的推移而增加，但与近皮质骨相比，其程度较低。我们的研究结果清楚地显示了种植体周围新骨形成质量的变化，这些变化与矿化有关。
The purpose of this study was to investigate changes in bone quality associated with the mineralization of new bone forming around implants. Eight 18-week-old male New Zealand white rabbits and implants fabricated from Ti-15%Zr-4%Nb-4% Ta alloy were used in the study. Characteristics of new bone formed around implants at 4 and 8 weeks and cortical bone were compared using X-ray photoelectron spectroscopy (XPS) for elemental qualitative and quantitative analysis, polarized microscopy (PM) for histological examination, and Fourier transform infrared Imaging (FTIR Imaging) to characterize molecular distributions in new bone and cortical bone. Results from XPS analysis showed changes in the chemical bonding in new bone over time (4 weeks vs. 8 weeks). PM showed changes in collagen birefringence, orientation and intensity in new bone over time, becoming more similar to those of cortical bone after 8 weeks. FTIR Imaging showed that in new bone close to cortical bone, amounts of PO43- and CO32- ions (representing mineral or inorganic components), and amounts of amide I (representing the organic component) increased over time as mineralization progressed. In new bone more distant from cortical bone, PO43-, CO32-, and amide I distributions also increased over time, but to a lesser extent to that observed near cortical bone. Our findings clearly showed changes in the quality of new bone forming around implants, and these changes were related to mineralization.