Insights into complex infection by two Pectobacterium species causing potato blackleg and soft rot.
Insights into complex infection by two Pectobacterium species causing potato blackleg and soft rot.
复制标题
DOI：
10.1016/j.micres.2022.127072
复制
发表时间：
2022-05
影响因子：
6.7
通讯作者：
Zhenhe Su;Xiaomeng Liu;Qinggang Guo;Lifeng Xuan;Xiuyun Lu;Li-hong Dong;Xiaoyun Zhang;Peipei Wang;Weisong Zhao;Yuanhang Qu;Shezeng Li;P. Ma
中科院分区：
文献类型：
--
作者：
Zhenhe Su;Xiaomeng Liu;Qinggang Guo;Lifeng Xuan;Xiuyun Lu;Li-hong Dong;Xiaoyun Zhang;Peipei Wang;Weisong Zhao;Yuanhang Qu;Shezeng Li;P. Ma
Pectobacteriumspp。是马铃薯黑腿病和软腐病的病原体。然而，不同胸腺杆菌混合感染的致病性之间的关系尚不清楚。在不同温度下。在这项研究中，两株果胶溶菌。从具有典型黑腿病症状的同一株马铃薯植株中分离得到，鉴定出asP。brasilienseandP。cartovorumby多位点序列分析（MLSA）、全基因组系统发育树构建、平均核苷酸识别（ANI）分析和数字DNA-DNA杂交（dDDH）。比较了两株病原菌对马铃薯块茎的致病力，比较了植物细胞壁降解酶（包括果胶酶、纤维素酶和蛋白酶）作为最重要的毒力因子。brasilienseBL-2 andP。cartovorumbl -4在28℃下。结果表明，p。胡萝卜素对马铃薯块茎的细胞壁降解酶活性较高，对马铃薯块茎的病害症状较p更严重。brasiliense。此外，p。carotovorumandP。随着温度的升高（20、25、28、32℃），brasiliense呈上升趋势。致病性在p。将cartovorum菌株BL-4与p共接种。brasiliensestrain BL-2，特别是当前者在初始时间表现出细菌数量优势时。本研究结果为了解不同种类胸腺杆菌混合感染的致病性提供了新的认识。对马铃薯黑腿病和软腐病的防治具有一定的指导意义。
Pectobacteriumspp. are causative agents of blackleg and soft rot of potato. However, little is known about the relationship between the pathogenicity of mixed infections of differentPectobacteriumspp. at different temperatures. In this study, two pectinolytic strains ofPectobacteriumspp. were isolated from the same potato plant with typical symptoms of blackleg and identified asP. brasilienseandP. carotovorumby multilocus sequence analysis (MLSA), whole-genome phylogenetic tree construction, average nucleotide identity (ANI) analysis and digital DNA–DNA hybridization (dDDH). Plant cell wall degrading enzyme, including pectinases, cellulases and proteases, as the most important virulence factors, as well as pathogenicity toward potato tuber, were compared between the strainsP. brasilienseBL-2 andP. carotovorumBL-4 at 28 ℃. The results showed thatP. carotovorumhad higher cell wall-degrading enzyme activities and brought more severe disease symptoms to potato tubers thanP. brasiliense. Moreover, the pathogenicity ofP. carotovorumandP. brasilienseincreased with increasing temperature (20, 25, 28, 32 ℃). The pathogenicity was more severe whenP. carotovorumstrain BL-4 was co-inoculated withP. brasiliensestrain BL-2, especially when the former exhibited an advantage in bacterial number at the initial time. The results of this study provide new insight for understanding the pathogenicity caused by mixed infections with different species ofPectobacteriumspp., and they may provide some guidance for controlling potato blackleg and soft rot.