lncRNA MTIJP Suppresses Biological Activities of Breast Cancer Cells in vitro and in vivo by Regulating the miRNA-214/RUNX3 Axis
lncRNA MTIJP Suppresses Biological Activities of Breast Cancer Cells in vitro and in vivo by Regulating the miRNA-214/RUNX3 Axis
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lncRNA MTIJP 通过调节 miRNA-214/RUNX3 轴在体外和体内抑制乳腺癌细胞的生物活性
DOI：
10.2147/ott.s241503
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发表时间：
2020-01-01
影响因子：
4
通讯作者：
Qu, Fei
中科院分区：
文献类型：
--
作者：
Ouyang, Qianwen;Cui, Yanru;Qu, Fei
简介：我们研究的目的是评估 MT1JP 在乳腺癌中的作用。材料和方法：出于临床目的，收集组织，并对 MT1JP 和 miRNA-214 基因表达进行相关性分析。采用体外研究，我们的研究以MDA-MB-231和MCF-7细胞系作为研究对象。采用集落、流式细胞术、TUNEL、transwell、粘附和伤口愈合实验来讨论细胞的生物学活性。在体内研究中，测量了肿瘤重量和体积，并通过 TUNEL 测定测量了细胞凋亡。通过Western blotting或免疫组化检测相关机制的蛋白。结果：与癌旁组织相比，MT1JP和miRNA-214基因表达存在显着差异（P
Introduction: The purpose of our research was to evaluate MT1JP in breast cancer.Material and Methods: For clinical purpose, tissues were collected, and a correlation analysis of MT1JP and miRNA-214 gene expressions was conducted. Using an in vitro study, MDA-MB-231 and MCF-7 cell lines were used as research objects in our research. Colony, flow cytometry, TUNEL, transwell, adhesion and wound healing assay were used to discuss the biological activities of the cells. In an in vivo study, tumor weight and volume were measured, and cell apoptosis was measured by TUNEL assay. The relative mechanism's proteins were evaluated by Western blotting or immunohistochemistry assay.Results: Compared with adjacent tissues, MT1JP and miRNA-214 gene expressions were significantly different (P