A major quantitative trait locus conferring subgynoecy in cucumber
A major quantitative trait locus conferring subgynoecy in cucumber
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DOI：
10.1007/s00122-015-2612-z
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发表时间：
2016-01-01
影响因子：
5.4
通讯作者：
Huang, Sanwen
中科院分区：
文献类型：
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作者：
Bu, Fengjiao;Chen, Huiming;Huang, Sanwen
黄瓜是性别决定研究的模式种，其产量与雌性化程度有关。亚雌核发育是一种高度女性化的性别形式，其遗传基础仍然难以捉摸。在这项研究中，遗传分析的F-2和BC1群体之间的杂交发展的亚雌蕊同株S-2 - 98和雌雄同株M95建议的数量性质的亚雌蕊异株。应用简单重复序列标记在BC1植株构建的雌雄同株和近雌同株群体之间鉴定了3个QTL：sg3.1、sg6.1和sg6.2。主要QTL sg3.1贡献了54.6%的表型变异，并且其存在通过亲本系和从BC6植物构建的亚雌蕊群之间的SNP谱的全基因组比较来证实。使用从SNP谱开发的基于PCR的标记，sg3.1被进一步限定为799 kb的基因组区域。揭示了亚雌核发育的遗传基础，为选育高产优良品种提供了理论依据。
Cucumber (Cucumis sativus L.) is a model species for sex determination studies, and its yield is associated with the degree of femaleness. Subgynoecy represents a sex form with a high degree of femaleness for which the genetic basis remains elusive. In this study, genetic analysis in the F-2 and BC1 populations developed from a cross between subgynoecious S-2-98 and monoecious M95 suggested a quantitative nature of subgynoecy. Application of simple sequence repeat markers between subgynoecious and monoecious bulks constructed from BC1 plants identified three QTLs: sg3.1, sg6.1, and sg6.2. The major QTL sg3.1 contributed to 54.6 % of the phenotypic variation, and its presence was confirmed by genome-wide comparison of SNP profiles between parental lines and a subgynoecious bulk constructed from BC6 plants. Using PCR-based markers developed from the SNP profile, sg3.1 was further delimited to a genomic region of 799 kb. The genetic basis of subgynoecy revealed here shall shed light on the development of elite cultivars with high yield potential.