Wenyang Huazhuo Tongluo formula, a Chinese herbal decoction, improves skin fibrosis by promoting apoptosis and inhibiting proliferation through down-regulation of survivin and cyclin D1 in systemic sclerosis.
Wenyang Huazhuo Tongluo formula, a Chinese herbal decoction, improves skin fibrosis by promoting apoptosis and inhibiting proliferation through down-regulation of survivin and cyclin D1 in systemic sclerosis.
复制标题
温阳化浊通络方是一种中药汤剂，通过下调系统性硬化症患者的生存素和细胞周期蛋白 D1 来促进细胞凋亡和抑制增殖，从而改善皮肤纤维化。
DOI：
10.1186/s12906-016-1056-6
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发表时间：
2016-02-20
影响因子：
--
通讯作者：
Ye S
中科院分区：
文献类型：
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作者：
Han L;Bian H;Ouyang J;Bi Y;Yang L;Ye S
纤维化是导致系统性硬化症(SSC)相关发病率的主要因素，而快速、进行性的皮肤受累预示着以后的死亡率。目前西医治疗系统性硬化症疗效不佳，而中药温阳化浊通络方在临床应用中显示出了惊人的抗纤维化效果。本研究旨在探讨温阳还五汤治疗系统性硬化症的抗纤维化作用机制。将原代培养的SSc患者皮损成纤维细胞暴露于WYHZTL或XAV939含药血清中，XAV939是tankyrase1/2和Wnt/β-catenin通路的小分子抑制剂。细胞计数试剂盒-8法和Annexin V FITC/PI凋亡试剂盒分别检测成纤维细胞的增殖和凋亡情况。逆转录-聚合酶链式反应(RT-PCR)和免疫印迹法检测细胞周期蛋白D1mRNA和蛋白表达水平。培养28、48、72h后，WYHZTL含药血清对成纤维细胞的增殖能力明显低于对照组，细胞凋亡率明显高于对照组，与XAV939组相近。WYHZTL含药血清与对照组相比，可下调细胞周期蛋白D1mRNA和蛋白表达水平。本研究证实了WYHZTL方对成纤维细胞的抗增殖和促凋亡作用，其作用机制可能与下调SSC中细胞周期蛋白D1mRNA和蛋白水平有关。
Fibrosis is a major contributor to systemic sclerosis (SSc)-related morbidity, and rapid, progressive skin involvement predicts later mortality. Western medicine therapies for SSc cannot produce satisfactory effects currently, while Traditional Chinese Medicine (TCM), such as the Wenyang Huazhuo Tongluo (WYHZTL) formula, a Chinese herbal decoction, has shown amazing anti-fibrosis efficacy on SSc in clinical applications. This study is aiming to investigate the anti-fibrotic mechanism of WYHZTL formula for the treatment of SSc. Fibroblasts from primary culture of skin lesions of SSc patients were exposed to rat medicated sera containing WYHZTL or XAV939, a small-molecule inhibitor of both tankyrase 1/2 and Wnt/β-catenin pathway. Cell counting kit-8 assay and Annexin V FITC/PI apoptosis kit were used to analyze cell proliferation and apoptosis in fibroblasts, respectively. Reverse transcription-polymerase chain reaction (RT-PCR) and western blotting were used to detect the mRNA and protein levels of cyclin D1 and survivin. After 28, 48 and 72 h of incubation, the proliferative ability of the fibroblasts cells was obviously reduced by the sera containing WYHZTL compared with that in the control group; the percentage of apoptotic cell population in the sera containing WYHZTL treated fibroblasts cells was significantly higher than that in those treated with the control sera, and was about similar to that in those treated with XAV939. The sera containing WYHZTL could down-regulate both mRNA and protein levels of cyclin D1 and survivin, compared with the control group. The present study demonstrates the antiproliferative and pro-apoptotic actions of WYHZTL formula against fibroblasts and the effect may be related to the down-regulation of mRNA and protein levels of cyclin D1 and survivin in SSc.