Expression of TRPC4 channel protein that interacts with NHERF-2 in rat descending vasa recta.
Expression of TRPC4 channel protein that interacts with NHERF-2 in rat descending vasa recta.
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DOI：
10.1152/ajpcell.00417.2004
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发表时间：
2005-04
期刊：
影响因子：
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通讯作者：
W. Lee‐Kwon;J. Wade;Zhong Zhang;T. Pallone;E. Weinman
中科院分区：
文献类型：
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作者：
W. Lee‐Kwon;J. Wade;Zhong Zhang;T. Pallone;E. Weinman
PDZ结构域衔接蛋白Na+/H+交换调节因子（NHERF）-2在肾髓质降直血管（DVR）中表达，尽管其功能尚未确定。瞬时受体电位通道（TRPC）TRPC 4和TRPC 5是转运Ca 2+的非选择性阳离子通道，最近被证明与NHERF蛋白复合。我们研究了TRPC 4和/或TRPC 5是否与DVR中的NHERF-2相关。RT-PCR显示TRPC 4和NHERF-2的mRNA，但没有TRPC 5或NHERF-1，在显微切割的DVR。免疫组织化学研究表明TRPC 4和NHERF-2蛋白在内皮细胞和周细胞中表达。这些蛋白共定位于DVR的一些细胞中。TRPC 4与来自肾髓质裂解物的NHERF-2共免疫沉淀，并且NHERF-2与TRPC 4共免疫沉淀。TRPC 5未检测到DVR与使用免疫组化或NHERF-2免疫沉淀。我们的结论是DVR周细胞和内皮细胞共表达TRPC 4和NHERF-2 mRNA和蛋白，这些蛋白共定位和免疫共沉淀，表明可能的物理协会。这些发现表明TRPC 4和NHERF-2可能在与Ca 2+信号相关的相互作用中发挥作用。
The PDZ domain adaptor protein Na+/H+ exchanger regulatory factor (NHERF)-2 is expressed in renal medullary descending vasa recta (DVR), although its function has not been defined. Transient receptor potential channels (TRPC) TRPC4 and TRPC5, nonselective cation channels that transport Ca2+, were recently demonstrated to complex with the NHERF proteins. We investigated whether TRPC4 and/or TRPC5 are associated with NHERF-2 in DVR. RT-PCR revealed mRNA for TRPC4 and NHERF-2, but not for TRPC5 or NHERF-1, in microdissected DVR. Immunohistochemical studies demonstrated expression of TRPC4 and NHERF-2 proteins in both the endothelial cells and pericytes. These proteins colocalized in some cells of the DVR. TRPC4 coimmunoprecipitated with NHERF-2 from renal medullary lysates, and NHERF-2 coimmunoprecipitated with TRPC4. TRPC5 was not detected in DVR with the use of immunohistochemistry or in NHERF-2 immunoprecipitates. We conclude that DVR pericytes and endothelia coexpress TRPC4 and NHERF-2 mRNA and protein and that these proteins colocalize and coimmunoprecipitate, indicating a possible physical association. These findings suggest that TRPC4 and NHERF-2 may play a role in interactions related to Ca2+ signaling.