An electronic pressure-meter nociception paw test for mice
An electronic pressure-meter nociception paw test for mice
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DOI：
10.1590/s0100-879x2004000300018
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发表时间：
2004-03-01
影响因子：
2.3
通讯作者：
Ferreira, S.H.
中科院分区：
文献类型：
--
作者：
Cunha, T.M.;Verri Jr., W.A.;Ferreira, S.H.
本研究的目的是描述和验证一种用于量化小鼠炎症伤害感觉强度的电子机械测试。电子压力计测试包括用与手持式力传感器相适应的聚丙烯移液管尖端戳足底区域，诱导动物后爪屈曲反射。该方法与经典的von Frey细丝实验进行了比较，后者在伤害性后爪屈曲反射后自动记录压力强度。利用电子压力计和von Frey丝检测卡拉胶诱导的高痛觉的时间与治疗的相互作用。在两个独立的实验中，电子压力计在检测小剂量的角叉菜胶（30杯）引起的伤害性感觉（高伤害性感觉）的增加方面比冯·弗雷丝更敏感。电子压力计检测非甾体类药物的抗痛觉作用呈剂量依赖性。腹腔注射吲哚美辛（1.8 ~ 15 mg/kg）、足底注射（30 ~ 300杯/爪）可抑制卡拉胶（100杯/爪）的过敏作用。电子压力计还检测前列腺素E-2 （PGE）的高痛觉作用(2)；10- 100ng)，呈剂量依赖性。双吡隆（160和320杯/只）可阻断PGE(2) （100 ng）的高痛觉作用，但足底注射吲哚美辛（300杯/只）不能阻断PGE(2)的作用。目前的结果证实了电子压力计在小鼠中的使用比冯弗雷丝更敏感。此外，它是一种客观定量的伤害性试验，用于评价抑制前列腺素合成（吲哚美辛）或直接阻断正在进行的高伤害性感觉（双吡酮）的抗炎镇痛药物的外周抗伤害性作用。
The aim of the present investigation was to describe and validate an electronic mechanical test for quantification of the intensity of inflammatory nociception in mice. The electronic pressure-meter test consists of inducing the animal hindpaw flexion reflex by poking the plantar region with a polypropylene pipette tip adapted to a hand-held force transducer. This method was compared to the classical von Frey filaments test in which pressure intensity is automatically recorded after the nociceptive hindpaw flexion reflex. The electronic pressure-meter and the von Frey filaments were used to detect time versus treatment interactions of carrageenin-induced hypernociception. In two separate experiments, the electronic pressure-meter was more sensitive than the von Frey filaments for the detection of the increase in nociception (hypernociception) induced by small doses of carrageenin (30 mug). The electronic pressure-meter detected the antinociceptive effect of nonsteroidal drugs in a dose-dependent manner. Indomethacin administered intraperitoneally (1.8-15 mg/kg) of intraplantarly (30-300 mug/ paw) prevented the hypersensitive effect of carrageenin (100 mug/ paw). The electronic pressure-meter also detected the hypernociceptive effect of prostaglandin E-2 (PGE(2); 10-100 ng) in a dose-dependent manner. The hypernociceptive, effect of PGE(2) (100 ng) was blocked by dipyrone (160 and 320 mug/paw) but not by intraplantar administration of indomethacin (300 mug/paw). The present results validate the use of the electronic pressure-meter as more sensitive than the von Frey filaments in mice. Furthermore, it is an objective and quantitative nociceptive test for the evaluation of the peripheral antinociceptive effect of anti-inflammatory analgesic drugs, which inhibit prostaglandin synthesis (indomethacin) or directly block the ongoing hypernociception (dipyrone).