Residual human immunodeficiency virus (HIV) type 1 RNA and DNA in lymph nodes and HIV RNA in genital secretions and in cerebrospinal fluid after suppression of viremia for 2 years
Residual human immunodeficiency virus (HIV) type 1 RNA and DNA in lymph nodes and HIV RNA in genital secretions and in cerebrospinal fluid after suppression of viremia for 2 years
复制标题
DOI：
10.1086/319864
复制
发表时间：
2001-05-01
影响因子：
6.4
通讯作者：
Wong, JK
中科院分区：
文献类型：
--
作者：
Günthard, HF;Havlir, DV;Wong, JK
在接受强效抗逆转录病毒治疗的人类免疫缺陷病毒（HIV）感染患者中，很大一部分患者存在残余病毒复制。为了确定这种病毒的来源，我们检测了28名接受茚地那韦、齐多夫定和拉米夫定治疗少于或等于2.5年的患者淋巴组织中HIV RNA和DNA水平以及血清、脑脊液（CSF）和生殖器分泌物中病毒RNA水平。在所有淋巴结中均可检测到HIV RNA和DNA。相比之下，治疗2年后，在23例生殖器分泌物中的20例或13例CSF样本中的任何一例中均未检测到HIV RNA。来自4名患者的血浆和淋巴结的HIV包膜序列数据显示序列差异，这表明3名患者存在不同程度的残留病毒复制，1名患者不存在。在接受强效抗逆转录病毒治疗的患者中，最大的病毒负荷可能仍然是淋巴组织，而不是中枢神经系统或泌尿生殖系统。
Residual viral replication persists in a significant proportion of human immunodeficiency virus (HIV)-infected patients receiving potent antiretroviral therapy. To determine the source of this virus, levels of HIV RNA and DNA from lymphoid tissues and levels of viral RNA in serum, cerebrospinal fluid (CSF), and genital secretions in 28 patients treated for less than or equal to2.5 years with indinavir, zidovudine, and lamivudine were examined. Both HIV RNA and DNA remained detectable in all lymph nodes. In contrast, HIV RNA was not detected in 20 of 23 genital secretions or in any of 13 CSF samples after 2 years of treatment. HIV envelope sequence data from plasma and lymph nodes from 4 patients demonstrated sequence divergence, which suggests varying degrees of residual viral replication in 3 and absence in 1 patient. In patients receiving potent antiretroviral therapy, the greatest virus burden may continue to be in lymphoid tissues rather than in central nervous system or genitourinary compartments.