Zingiber officinale Protects HaCaT cells and C57BL/6 Mice from Ultraviolet B-Induced Inflammation
Zingiber officinale Protects HaCaT cells and C57BL/6 Mice from Ultraviolet B-Induced Inflammation
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DOI：
10.1089/jmf.2009.1239
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发表时间：
2010-06-01
影响因子：
2.4
通讯作者：
Kim, Sun Yeou
中科院分区：
文献类型：
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作者：
Guahk, Gui-Hee;Ha, Sang Keun;Kim, Sun Yeou
紫外线是一种物理致癌物质，来自阳光的紫外线辐射对人体皮肤有负面影响。UVB诱导的炎症与多种细胞(包括角质形成细胞)中各种炎性细胞因子/趋化因子的过度诱导有关。本研究旨在探讨生姜水提物、姜辣素和姜辣素对中波紫外线诱导的人角质形成细胞株HaCaT和C57BL/6小鼠皮肤损伤的抗炎作用。为了测试一种有效的化合物，以防止紫外线损伤的皮肤炎症，我们制备了生姜根茎的水提取物，并检测了药用植物姜辣素和姜辣素对紫外线照射的HaCaT细胞的细胞活力和细胞因子/趋化因子产生的影响。我们还通过苏木精-伊红染色和细胞因子测定，研究了这些发现在C57BL/6小鼠体内的相关性。苍耳子、姜辣素和雪茄酚的水提取物有效地抑制了UVB照射的HaCaT细胞中细胞因子的产生。药用泽兰治疗可减轻UVB诱导的小鼠真皮的增殖、白细胞的渗透和血管的扩张。中草药、姜辣素和姜辣素显示出潜在的抗炎作用，可以保护皮肤免受UVB辐射损伤。
Ultraviolet (UV) light is a physical carcinogen, and UV irradiation from sunlight has negative effects on human skin. UVB-induced inflammation is linked to excessive induction of various inflammatory cytokines/chemokines in many types of cells, including keratinocytes. The purpose of this study was to investigate the anti-inflammatory effect of water extract of Zingiber officinale, gingerol, and shogaol on UVB-induced skin damage in the human keratinocyte cell line HaCaT and C57BL/6 mice. To test for an effective compound to protect against inflammation in UV-damaged skin, we prepared a water extract of ginger rhizomes and examined the effects of Z. officinale, gingerol, and shogaol on cell viability and cytokine/chemokine production in UV-irradiated HaCaT cells. We also investigated the in vivo relevance of these findings in C57BL/6 mice using hematoxylin and eosin staining and cytokine measurements. A water extract of Z. officinale, gingerol, and shogaol inhibited production of cytokines in UVB-irradiated HaCaT cells effectively. Treatment with Z. officinale attenuated UVB-induced hyperplasia, infiltration of leukocytes, and dilation of blood vessels in the dermis of mice. Z. officinale, gingerol, and shogaol show potential as anti-inflammatory agents to protect skin against UVB irradiation damage.