TIME-DEPENDENT SYNERGISTIC INTERACTIONS BETWEEN THE VASODILATOR NEUROPEPTIDE, CALCITONIN GENE-RELATED PEPTIDE (CGRP) AND MEDIATORS OF INFLAMMATION
TIME-DEPENDENT SYNERGISTIC INTERACTIONS BETWEEN THE VASODILATOR NEUROPEPTIDE, CALCITONIN GENE-RELATED PEPTIDE (CGRP) AND MEDIATORS OF INFLAMMATION
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DOI：
10.1111/j.1476-5381.1991.tb09819.x
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发表时间：
1991-06-01
影响因子：
7.3
通讯作者：
WILLIAMS, TJ
中科院分区：
文献类型：
--
作者：
BUCKLEY, TL;BRAIN, SD;WILLIAMS, TJ
1.在家兔背部皮肤中，观察了长效神经肽血管扩张剂降钙素基因相关肽（CGRP）在增强水肿形成和中性粒细胞聚集中的作用。 通过皮内（i.d.）注射 然后通过定量放射性标记技术测量水肿形成和中性粒细胞蓄积。2 CGRP（每个部位1 × 10（-11）mol）增强酵母聚糖活化血浆（用作C5 a des Arg的来源）、N-甲酰基-甲硫氨酰-亮氨酰-苯丙氨酸（FMLP）和白三烯B4（LTB 4）诱导的中性粒细胞蓄积。 相反，单独注射CGRP时不会诱导中性粒细胞蓄积。3由一系列化学性质不同的微血管通透性增加介质诱导的水肿形成;缓激肽、血小板活化因子（PAF）、FMLP和酵母聚糖活化血浆; i.d. 4.当i.d.后30-60分钟测量水肿时，CGRP也显著增强了由酵母多糖活化的血浆和FMLP而不是缓激肽和PAF诱导的水肿形成。注射 在i.d.后30-60分钟测量由酵母聚糖活化血浆诱导的水肿的增强。在存在作用较短的前列腺素类血管扩张剂前列环素（PGI 2，3 × 10（-10）mol/位点）的情况下，没有观察到注射。5这些结果表明，CGRP由于其持续的血管扩张活性，可能对炎症条件下的中性粒细胞积聚和水肿形成具有延长的增强作用。
1 The action of the long lasting neuropeptide vasodilator, calcitonin gene-related peptide (CGRP), in potentiating oedema formation and neutrophil accumulation was investigated in the dorsal skin of the rabbit, in vivo. Combinations of agents under test were administered by intradermal (i.d.) injection. Oedema formation and neutrophil accumulation were then measured by quantitative radiolabel techniques.2 CGRP (1 x 10(-11) mol per site) potentiated neutrophil accumulation induced by zymosan activated plasma, (used as a source of C5a des Arg), N-formyl-methionyl-leucyl-phenylalanine (FMLP) and leukotriene B4 (LTB4). In contrast CGRP did not induce neutrophil accumulation when injected alone.3 Oedema formation induced by a series of chemically distinct mediators of increased microvascular permeability; bradykinin, platelet activating factor (PAF), FMLP and zymosan-activated plasma; measured 0-30 min after i.d. injection was potentiated by CGRP (1 x 10(-11) mol per site).4 Oedema formation induced by zymosan activated plasma and FMLP but not bradykinin and PAF, was also significantly potentiated by CGRP when oedema was measured 30-60 min after i.d. injection. The potentiation of oedema induced by zymosan activated plasma measured 30-60 min after i.d. injection was not observed in the presence of the shorter acting prostanoid vasodilator prostacyclin (PGI2, 3 x 10(-10) mol per site).5 These results suggest that CGRP, as a consequence of its sustained vasodilator activity could have prolonged potentiating effects on neutrophil accumulation and oedema formation in inflammatory conditions.