Characterization and localization of adrenal nicotinic acetylcholine receptors: evidence that mAb35-nicotinic receptors are the principal receptors mediating adrenal catecholamine secretion.
Characterization and localization of adrenal nicotinic acetylcholine receptors: evidence that mAb35-nicotinic receptors are the principal receptors mediating adrenal catecholamine secretion.
复制标题
肾上腺烟碱乙酰胆碱受体的特征和定位：mAb35烟碱受体是介导肾上腺儿茶酚胺分泌的主要受体的证据。
DOI：
10.1046/j.1471-4159.1996.66041454.x
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发表时间：
1996
影响因子：
4.7
通讯作者：
McKay,DB
中科院分区：
文献类型：
--
作者：
Gu,H;Wenger,BW;Lopez,I;McKay,SB;Boyd,RT;McKay,DB
Adrenal chromaffin cells contain at least two subtypes of nicotinic acetylcholine receptors (nAChRs). These studies were designed to identify and characterize the subtype of nAChR mediating adrenal catecholamine release using the monoclonal antibody mAb35, which recognizes the α‐subunit of muscle nAChRs and cross‐reacts with some neuronal nAChRs. Immunocytochemical studies demonstrated that mAb35 interacts with specific sites on cultured chromaffin cells. Pretreatment with mAb35 reduced nAChR‐stimulated catecholamine release (IC50of ∼10 nM). mAb35 had no effects on release stimulated through non‐nAChR mechanisms. Unlike agonist‐induced nAChR desensitization, the mAb35‐induced reduction in nAChR‐mediated secretion developed slowly. Although not immediately reversible, nAChR‐stimulated release recovered after mAb35 removal. However, unlike recovery from agonist pretreatment, recovery from mAb35 pretreatment was relatively slow and was partially blocked by vinblastine. Hybridization of adrenal chromaffin RNA with a rat α3 cDNA revealed two strong bands and two fainter bands: two higher‐molecular‐weight bands, 6.9 and 8.5 kb; a strong band of 3.2 kb; and a lower amount of a 2.3‐kb RNA. With recovery of nAChR function after agonist or mAb35 treatment, no significant effects on α3 subunit mRNA levels were seen. In summary, these studies demonstrate the presence of mAb35‐nAChRs on adrenal chromaffin cells and provide evidence that these receptors represent the major population that regulates secretory events in adrenal chromaffin cells.
