Metabolic engineering of cyanobacteria for 1-butanol production from carbon dioxide
Metabolic engineering of cyanobacteria for 1-butanol production from carbon dioxide
复制标题
DOI：
10.1016/j.ymben.2011.04.004
复制
发表时间：
2011-07-01
影响因子：
8.4
通讯作者：
Liao, James C.
中科院分区：
文献类型：
--
作者：
Lan, Ethan I.;Liao, James C.
直接从二氧化碳生产化学品和燃料是解决能源和环境问题的一种有吸引力的方法。1-丁醇是一种化学原料和潜在的燃料，在天然梭状芽孢杆菌和各种工程宿主中都是通过碳水化合物发酵生产的。为了从二氧化碳中生产正丁醇，我们将一条改良的依赖CoA的正丁醇生产途径转移到蓝藻细长聚球藻PCC7942中。我们通过染色体整合和基因表达证明了该途径中各酶的活性。特别是，利用NADH作为还原动力的齿密螺旋体反式烯基辅酶A还原酶(Ter)用于将巴豆酰辅酶A还原为丁酰辅酶A，以代替乙酰丁酸梭菌丁酰辅酶A脱氢酶，从而绕过了梭状芽孢杆菌铁氧还蛋白的需要。多组氨酸TAG的加入增加了Ter的总活性，并导致了更高的1-丁醇产量。氧的去除是该生物合成正丁醇的一个重要因素。这一结果是第一次自养生产1-丁醇。(C)2011 Elsevier Inc.保留所有权利。
Production of chemicals and fuels directly from CO2 is an attractive approach to solving the energy and environmental problems. 1-Butanol, a chemical feedstock and potential fuel, has been produced by fermentation of carbohydrates, both in native Clostridium species and various engineered hosts. To produce 1-butanol from CO2, we transferred a modified CoA-dependent 1-butanol production pathway into a cyanobacterium, Synechococcus elongatus PCC 7942. We demonstrated the activity of each enzyme in the pathway by chromosomal integration and expression of the genes. In particular, Treponema denticola trans-enoyl-CoA reductase (Ter), which utilizes NADH as the reducing power, was used for the reduction of crotonyl-CoA to butyryl-CoA instead of Clostridium acetobutylicum butyryl-CoA dehydrogenase to by-pass the need of Clostridial ferredoxins. Addition of polyhistidine-tag increased the overall activity of Ter and resulted in higher 1-butanol production. Removal of oxygen is an important factor in the synthesis of 1-butanol in this organism. This result represents the first autotrophic 1-butanol production. (C) 2011 Elsevier Inc. All rights reserved.