Gene expression profiling of the whitefly (Bemisia tabaci) Middle East - Asia Minor 1 feeding on healthy and Tomato yellow leaf curl China virus-infected tobacco
Gene expression profiling of the whitefly (Bemisia tabaci) Middle East - Asia Minor 1 feeding on healthy and Tomato yellow leaf curl China virus-infected tobacco
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中东-小亚细亚 1 号烟粉虱 (Bemisia tabaci) 取食健康和番茄黄化曲叶中国病毒感染的烟草后的基因表达谱
DOI：
10.1111/j.1744-7917.2010.01386.x
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发表时间：
2011-02-01
期刊：
影响因子：
4
通讯作者：
Wang, Xiao-Wei
中科院分区：
文献类型：
--
作者：
Li, Jun-Min;Ruan, Yong-Ming;Wang, Xiao-Wei
双生病毒仅由粉虱（烟粉虱）以循环、非繁殖方式传播。菜豆花叶病毒对烟粉虱媒介的影响是复杂的，既有直接作用，也有间接作用。然而，这些影响的分子机制知之甚少，健康和病毒感染的植物上的粉虱的转录谱尚未进行研究。利用抑制性消减杂交技术，研究了中东-小亚细亚1号烟粉虱取食健康烟草和感染中国番茄黄化曲叶病毒（TYLCCNV）烟草后的差异表达基因。从正向cDNA文库中，获得了124个差异表达的表达序列标签（ESTs），这些标签代表了以感染烟草为食的烟粉虱中上调的基因。从反向文库中分离到112个表达下调的EST。在上调的基因中，我们确定了几个可能参与粉虱和TYLCCNV之间直接相互作用的基因，包括似乎参与消除外源蛋白的26/29-kDa蛋白酶，介导几种病毒进入宿主细胞的硫酸乙酰肝素蛋白聚糖和粉虱的次级内共生细菌立克次体的两个基因。此外，我们还鉴定了一些与代谢、转录和翻译有关的基因，这些基因可能是TYLCCNV通过寄主植物间接影响烟粉虱的结果。总的来说，我们的研究结果表明，TYLCCNV感染的烟草通过直接和间接的相互作用改变了烟粉虱的基因表达谱。本研究揭示了烟粉虱-TYLCCNV-烟草互作的相关基因，为进一步研究这一复杂系统提供了有用的信息。
Begomoviruses are exclusively transmitted by whitefly (Bemisia tabaci) in a circulative, non-propagative manner. The influences of begomoviruses on whitefly vector are complex with both direct and indirect effects. However, the molecular mechanisms underlying these effects are poorly understood and the transcriptional profiles of whitefly on healthy and virus-infected plants have not yet been studied. Using suppression subtractive hybridization, we investigated the differentially expressed genes in whitefly Middle East - Asia Minor 1 feeding on healthy and Tomato yellow leaf curl China virus (TYLCCNV) infected tobacco. From the forward cDNA library, 124 differentially expressed expression sequence tags (ESTs) were obtained which represent up-regulated genes in the whiteflies feeding on the infected tobacco. From the reverse library, 112 ESTs were isolated which represent down-regulated genes. Among the up-regulated genes, we identified several genes that are probably involved in direct interaction between whitefly and TYLCCNV, including a 26/29-kDa proteinase that appears to participate in the elimination of foreign proteins, heparan sulfate proteoglycan which mediates the entry of several viruses into host cells and two genes of Rickettsia-a secondary endosymbiotic bacterium of whitefly. In addition, we identified a number of genes involved in metabolism, transcription and translation which might be the result of indirect effects of TYLCCNV on the whitefly via host plants. Collectively, our results suggest that TYLCCNV-infected tobacco changes the gene expression profiles of whitefly via both direct and indirect interactions. This study revealed a number of genes involved in whitefly-TYLCCNV-tobacco interactions and provided useful information for future study on this complex system.