PLC Methods for Determination of D-Aspartate and N-methyl-D-Aspartate
PLC Methods for Determination of D-Aspartate and N-methyl-D-Aspartate
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DOI：
10.1007/978-1-61779-331-8_16
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发表时间：
2012-01-01
期刊：
影响因子：
--
通讯作者：
Tsesarskaia, Mara
中科院分区：
文献类型：
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作者：
Fisher, George H.;Tsesarskaia, Mara
D-氨基酸是天然存在的L-氨基酸的立体异构体或光学异构体，因此具有相同的化学结构，但其生物/生理特性可能不同。直到半个世纪前，D-氨基酸还被认为是仅在微生物中发现的非天然物质。然而，分析仪器和方法的改进表明，D-氨基酸存在于包括人类在内的无脊椎动物和脊椎动物中，并且它们具有重要的生理功能。 D-天冬氨酸 (D-Asp) 及其甲基化形式 N-甲基-D-天冬氨酸 (NMDA) 在许多物种中具有神经内分泌特性。已经开发出多种通过高效液相色谱 (HPLC) 测定氨基酸 D- 和 L- 对映体的方法。我们在这里报告了用于特异性测定生物组织中 D-Asp 和 NMDA 的改进 HPLC 方法。
D-Amino acids are stereoisomers or optical isomers of naturally occurring L-amino acids and thus possess the same chemical structure, but may differ in their biological/physiological properties. Until a half century ago, D-amino acids had been considered to be unnatural substances found only in microorganisms. However, improvements in analytical instruments and methods have revealed that D-amino acids are present in invertebrates and vertebrates, including humans, and that they possess important physiological functions. D-Aspartate (D-Asp) and its methylated form N-methyl-D-aspartate (NMDA) possess neuroendocrine properties in many species. Several methods have been developed for determination of D- and L-enantiomers of amino acids by high performance liquid chromatography (HPLC). We report here improved HPLC methods for the specific determination of D-Asp and NMDA in biological tissues.