Screen Printing Tissue Models Using Chemically Cross-Linked Hydrogel Systems: A Simple Approach To Efficiently Make Highly Tunable Matrices
Screen Printing Tissue Models Using Chemically Cross-Linked Hydrogel Systems: A Simple Approach To Efficiently Make Highly Tunable Matrices
复制标题
使用化学交联水凝胶系统丝网印刷组织模型：一种有效制作高度可调基质的简单方法
DOI：
10.1021/acsbiomaterials.1c00902
复制
发表时间：
2021
影响因子：
5.8
通讯作者：
Lavik, Erin
中科院分区：
文献类型：
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作者：
Pandala, Narendra;LaScola, Michael A.;Tang, Yanchun;Bieberich, Maria;Korley, LaShanda T.J.;Lavik, Erin
体外模型为药物筛选和理解对应于不同条件的各种细胞机制提供了良好的起点。3D培养已经引起了人们的极大兴趣，以更好地模拟体内微环境并克服2D单层培养的局限性。我们先前报道了一种基于丝网印刷过程的技术，使用明胶作为生物墨水来图案化活的哺乳动物细胞。尽管明胶是一种具有各种组织工程应用的廉价支架材料，但它可能不是为细胞提供各种机械和化学线索的理想水凝胶材料。在本文中，我们讨论了两种合成的化学交联的水凝胶系统的合成和表征的基础上聚（乙二醇）（PEG）和聚-L-赖氨酸（PLL）被用作生物油墨在丝网印刷过程中。这些水凝胶适合作为丝网印刷工艺的生物油墨，并作为裸骨材料，可以机械调节和化学增强，为细胞创造合适的体外微环境。本文介绍了水凝胶系统的合成，力学测试和表征及其在丝网印刷过程中的应用。
In vitro models provide a good starting point for drug screening and understanding various cellular mechanisms corresponding to different conditions. 3D cultures have drawn significant interest to mimic the in vivo microenvironment better and overcome the limitations of the 2D monolayered cultures. We previously reported a technique based on the screen printing process to pattern live mammalian cells using gelatin as the bioink. Even though gelatin is an inexpensive scaffolding material with various tissue engineering applications, it might not be the ideal hydrogel material to provide various mechanical and chemical cues to the cells. In this paper, we discuss the synthesis and characterization of two synthetic chemically cross-linked hydrogel systems based on poly(ethylene glycol) (PEG) and poly-l-lysine (PLL) to be used as the bioink in the screen printing process. These hydrogels are suitable as the bioinks for the screen printing process and serve as the barebone materials that can be tuned mechanically and augmented chemically to create a suitable in vitro microenvironment for the cells. This paper presents the synthesis, mechanical testing, and characterization of the hydrogel systems and their applications in the screen printing process.