Gene Expression Profiles in Response to Salt Stress in Hibiscus Tiliaceus
Gene Expression Profiles in Response to Salt Stress in Hibiscus Tiliaceus
复制标题
木槿响应盐胁迫的基因表达谱
DOI：
10.1007/s11105-010-0267-0
复制
发表时间：
2011-09-01
影响因子：
2.1
通讯作者：
Shi, Suhua
中科院分区：
文献类型：
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作者：
Yang, Guili;Zhou, Renchao;Shi, Suhua
黄槿是一种红树林植物，是研究耐盐性和适应性的理想植物，因为它可以栖息在内陆和沿海生境中。在本研究中，我们使用全长 cDNA 微阵列探索了 H. tiliaceus 在盐胁迫下的表达谱。在 H. tiliaceus 中鉴定出 486 个盐响应性单基因；其中224个与拟南芥具有较高的序列相似性。已知许多已鉴定的基因对盐胁迫有反应。此外，我们还研究了 H. tiliaceus 在盐胁迫下的生理表现。生理分析显示K+/Na+比值下降，对黄鳅光合作用产生负面影响。我们的研究表明，为了在高盐度潮间带环境下生存，黄鳅进化出了自己的机制，通过与基因转录、信号传导、下游细胞运输和解毒途径相关的基因的协调参与来重新获得离子和渗透稳态。
Hibiscus tiliaceus, a mangrove associate, is an ideal plant for studying salt tolerance and adaptation since it can inhabit both inland and littoral habitats. In this study, we explored the expression profiles of H. tiliaceus under salt stress using a full-length cDNA microarray. Four hundred eighty-six salt-responsive unigenes were identified in H. tiliaceus; 224 of which had high sequence similarity to Arabidopsis. Many genes identified are known to be salt-stress responsive. Furthermore we examined the physiological performance of H. tiliaceus under salt stress. Physiological analysis displayed decrease in ratio of K+/Na+ and negative influence on photosynthesis of H. tiliaceus. Our study indicated that to survive under high salinine intertidal environments, H. tiliaceus evolved its own mechanisms to re-gain both ionic and osmotic homeostasis through coordinated engagement of genes associated with gene transcription, signaling, and down-stream cell transport and detoxification pathways.