Tilt-angle measurement of a sample stage using a capacitive liquid-based inclinometer
Tilt-angle measurement of a sample stage using a capacitive liquid-based inclinometer
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使用电容式液基倾角仪测量样品台的倾斜角
DOI：
10.1093/jmicro/dfs034
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
et al
中科院分区：
文献类型：
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作者：
M.Matoba;et al
在层析成像重建中，投影图像的对齐是决定重建三维图像质量的关键过程。最流行的校准方法是基于标记的校准，它通常使用添加到标本中的胶体金颗粒（称为基准标记）来计算倾斜系列中每个投影图像的坐标。然而，当每个图像只包含少量基准标记时，这种方法是无效的。因此，在校准所需的所有参数中，我们将重点放在倾斜角度上，并试图直接测量它，以检查获得的角度是否足够精确，可以进行层析成像重建。我们表明，使用市售的电容式液体倾斜仪测量的倾斜角比电子显微镜监视器的读数更精确，并且它可以导致类似于基于标记的校准方法获得的质量的3D重建。
Alignment of projection images in tomographic reconstruction is a critical process that governs the quality of the reconstructed three-dimensional (3D) image. The most popular alignment method is the marker-based alignment, which typically uses colloidal gold particles added to the specimen (called fiducial markers) to calculate the coordinates of each projection image in the tilt series. This method, however, is not effective when each image contains only a small number of fiducial markers. Therefore, of all the parameters required for alignment, we focussed on the tilt angle and attempted to gage it directly in order to examine whether the acquired angle is accurate enough to perform tomographic reconstruction. We showed that the tilt angle measured using a commercially available capacitive liquid-based inclinometer is more precise than the reading from the monitor of the electron microscope and that it can lead to 3D reconstructions of quality similar to those obtained by the marker-based alignment method.