Reserpine modulates serotonin transporter mRNA levels in the rat brain.
Reserpine modulates serotonin transporter mRNA levels in the rat brain.
复制标题
利血平调节大鼠大脑中的血清素转运蛋白 mRNA 水平。
DOI：
10.1016/s0024-3205(98)00534-7
复制
发表时间：
1999
期刊：
影响因子：
6.1
通讯作者：
Tejani-Butt,SM
中科院分区：
文献类型：
--
作者：
Xiao,Q;Pawlyk,A;Tejani-Butt,SM
尽管最近在确定中枢5-羟色胺（5-HT）功能方面取得了进展，但对5-羟色胺神经传递的控制和调节的基本方面仍然不清楚。由于5-羟色胺转运体（5-HTT）参与终止从突触前神经末梢释放的5-HT的作用，因此5-HTT的调节可能是控制突触间隙5-HT神经传递的重要步骤。本研究探讨了利血平给药对大鼠脑内5-HTT基因表达以及色氨酸羟化酶（TPH）表达的影响。将雄性Sprague-Dawley大鼠用利血平（10 mg/kg，i. p.）并在注射后8小时、3天、7天或21天处死。对照组大鼠注射生理盐水，并在注射后8 h或21 d处死。解剖中脑区域，分离RNA并使用北方印迹法探测5-HTT表达。使用Super-Anova分析数据，然后进行事后Dunnett检验。虽然5-HTT的mRNA水平在利血平给药后8小时没有变化，但在第3天和第7天观察到显著降低（F = 10; p < 0.0001）。5-HTT mRNA水平在21天时恢复到对照水平。相反，TPH表达在所有检查的时间点均未改变。这项研究的结果提供了有用的信息，5-HTT可能发挥的作用，在突触的5-HT神经传递的稳态控制。
Despite recent advances in determining central serotonin (5-HT) function, the basic aspects by which serotonin neurotransmission is controlled and regulated are still not understood. Since the serotonin transporter (5-HTT) is involved in terminating the action of 5-HT that is released from the presynaptic nerve terminal, the regulation of 5-HTT may be an important step in controlling 5-HT neurotransmission at the synaptic cleft. The present study investigated the effects of reserpine administration on 5-HTT gene expression as well as on tryptophan hydroxylase (TPH) expression in the rat brain. Male Sprague-Dawley rats were injected with reserpine (10 mg kg , i.p.) and sacrificed at 8 h, 3 days, 7 days or 21 days after the injection. Control rats were injected with saline and sacrificed either at 8 h or 21 days after the injection. The midbrain region was dissected, RNA was isolated and probed for 5-HTT expression using Northern Blotting. Data were analyzed using Super-Anova followed by posthoc Dunnett's test. While mRNA levels for 5-HTT were unchanged at 8 h after reserpine, a significant decrease was noted at 3 days and 7 days (F = 10; p < 0.0001). 5-HTT mRNA levels returned to control levels by 21 days. In contrast, TPH expression was unaltered at all time points examined. The results of this study provide useful information regarding the role that the 5-HTT may be playing in the homeostatic control of 5-HT neurotransmission at the synapse.