Kinetic and energetic parameters of imipramine binding to monoclonal antibodies as measured by fluorescence spectroscopy.
Kinetic and energetic parameters of imipramine binding to monoclonal antibodies as measured by fluorescence spectroscopy.
复制标题
通过荧光光谱测量丙咪嗪与单克隆抗体结合的动力学和能量参数。
DOI：
10.1089/hyb.1992.11.301
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Linthicum,DS
中科院分区：
文献类型：
--
作者：
Gabler,D;Mandal,C;Harrington,C;Adamczyk,M;Linthicum,DS
Monoclonal antibodies which bind small drugs are useful for the study of the interactive forces involved in antibody-ligand complexation. Detailed understanding of these supramolecular forces requires a careful examination of structural and thermodynamic parameters of the interacting molecules. Fluorescence spectroscopy techniques are very useful in this regard. We report here, the kinetic and energetic parameters of four monoclonal antibodies made against the tricyclic antidepressant imipramine. These monoclonal antibodies were found to possess high to very high binding affinity constants, ranging from 107to 1010M-1, and caused fluorescence quenching or enhancement of a fluorescein labelled imipramine. The dissociation rates of the fluorescent ligand from the complexes were measured at different temperatures in order to provide some insight regarding the kinetic and energetic (thermodynamic) parameters of the antibody-ligand binding interactions.
