Plastic components affect the activation of the aryl hydrocarbon and the androgen receptor
Plastic components affect the activation of the aryl hydrocarbon and the androgen receptor
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DOI：
10.1016/j.tox.2007.12.028
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发表时间：
2008-04-18
期刊：
影响因子：
4.5
通讯作者：
Bonefeld-Jorgensen, Eva C.
中科院分区：
文献类型：
--
作者：
Kruger, Tanja;Long, Manhai;Bonefeld-Jorgensen, Eva C.
酚类和增塑剂广泛用于塑料工业、食品包装以及赋予通常坚硬的塑料医疗器械和儿童玩具以柔软性和灵活性。利用荧光素酶报告基因分析方法，研究了双酚A(BPA)、4-正-壬基酚(NNP)、4-叔辛基苯酚(TOP)、邻苯二甲酸二(2-乙基己基)酯(DEHP)、邻苯二甲酸二异辛酯(DINP)、邻苯二甲酸二异癸酯(DIDP)、邻苯二甲酸二辛酯(DNOP)、邻苯二甲酸二丁酯(DBP)、邻苯二甲酸丁酯(BBP)、4-氯-3-甲基苯酚(CMP)、2-苯基苯酚(2-PP)、2，4-二氯苯酚(DCP)、邻苯二甲酸二辛酯(DNOP)、邻苯二甲酸二丁酯(DBP)、邻苯二甲酸二丁酯(BBP)、邻苯二甲酸二异辛酯(DCP)、邻苯二甲酸二异辛酯(DDP)等化合物对芳烃受体(AhR)和雄激素受体(AR)的影响。间苯二酚和己二酸二(2-乙基己基)酯(DEHA)。两种受体均受BPA、NNP、BBP、CMP、DCP和间苯二酚的影响，DEHP、DIDP和DBP仅影响AhR，TOP和2-PP拮抗AR活性。利用浓度相加原理，在NOEC、LOEC和EC50/IC50处观察到两种受体的相加效应。我们的体外数据表明，混合物的效应取决于单一混合物的浓度、性质、效力和组成，在人体健康风险评估中也应考虑化合物的综合效应。(C)2008爱思唯尔爱尔兰有限公司。保留所有权利。
Phenols and plasticizers are widely used in the plastic industry, in food packaging and to impart softness and flexibility to normally rigid plastic medical devices and children's toys. The effects on the aryl hydrocarbon receptor (AhR) and the androgen receptor (AR) were assessed using luciferase reporter gene assays of the following compounds: bisphenol A (BPA), 4-n-nonylphenol (nNP), 4-tert-octylphenol (tOP), bis(2-ethylhexyl) phthalate (DEHP), di-isononyl phthalate (DINP), diisodecyl phthalate (DIDP), di-n-octyl phthalate (DNOP), dibutyl phthalate (DBP), benzyl butyl phthalate (BBP), 4-chloro-3-methylphenol (CMP), 2-phenylphenol (2-PP), 2,4-dichlorophenol (DCP), resorcinol and bis(2-ethythexyl) adipate (DEHA). Furthermore, a mixture of selected compounds was tested at the no-observed-effect concentration (NOEC), the low-observed-effect concentration (LOEC) and the half-maximum-effect/inhibitory concentration (EC50/IC50) of the single chemicals.Both receptors were affected by BPA, nNP, BBP, CMP, DCP and resorcinol whereas DEHP, DIDP and DBP affected only the AhR and tOP and 2-PP antagonised the AR activity.The mixture was composed of 6 compounds, of which one compound weakly induced the AhR but all compounds antagonized the AR activation. Using the concentration addition principle additive effects were observed at the NOEC, LOEC and EC50/IC50 for both receptors.Our in vitro data suggest that the effect of a mixture depends on the concentration, character, potency and composition of the single mixture compounds and that also the combined effects of the compounds should be taken into consideration for risk assessment of human health. (C) 2008 Elsevier Ireland Ltd. All rights reserved.